ASM10324-A633-100 | Kv3.2 Antibody Clinisciences

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kv3.2 Monoclonal Antibody, Clone S410-17 (ASM10324). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Kv3.2 Monoclonal Antibody (ASM10324) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Kv3.2 Antibody (D) Composite.
Western Blot analysis of Mouse, Rat Brain showing detection of ~80-100 kDa Kv3.2 protein using Mouse Anti-Kv3.2 Monoclonal Antibody, Clone S410-17 (ASM10324). Lane 1: MW Ladder. Lane 2: Mouse Brain. Lane 3: Rat Brain Membrane. Lane 4: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Kv3.2 Monoclonal Antibody (ASM10324) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~80-100 kDa. Other Band(s): ~30 kDa for Rat Brain .

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, IHC, ICC
Primary Accession	P22462
Other Accession	NP_631962.1
Host	Mouse
Isotype	IgG1
Reactivity	Human, Mouse, Rat
Clonality	Monoclonal
Description	Mouse Anti-Rat Kv3.2 Monoclonal IgG1
Target/Specificity	Detects ~80-100kDa. Varies due to post-translational modifications.
Other Names	Potassium voltage-gated channel subfamily C member 2 Antibody, Kcnc2 Antibody, KSHIIIA Antibody
Clone Names	S410-17
Immunogen	Fusion protein amino acids 474-613 (Cytoplasmic C-terminus) of rat Kv3.2a
Purification	Protein G Purified
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.1% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	A 1:100 dilution of SMC-492 was sufficient for detection of Kv3.2 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization	Cell Membrane

Kv3.2 Antibody