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Lactic acid [50-21-5]

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Referentie NB-64-67298-1g

Formaat : 1g

Merk : Neo Biotech

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Lactic acid

(Synonyms: 2-hydroxypropanoic acid) Copy Product Info

Synonyms: 2-hydroxypropanoic acid

Catalog No. TN6945 Copy Product Info

Lactic acid (2-hydroxypropanoic acid), a glycolysis end product, is a hydroxycarboxylic acid receptor 1 (HCAR1) activator (EC50=4.2-5 mM). Lactic acid can be used as a hemodynamic index in critically ill patients and has anti-tumor activity.

Cas No. 50-21-5

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:90%

Appearance:Liquid

Color:Transparent

Product Introduction

Lactic acid AI Summary

Lactic acid shows notable bioactivities, including cytotoxic effects on cancer cells with p53 mutations at a potency of 25.1 nM. It also demonstrates the ability to block Ebola virus entry, with potency levels ranging from 35.48 µM to 39.81 µM in one assay and from 35481.3 nM to 39810.7 nM in another. Additionally, when administered topically at concentrations of 2% and 4% over four weeks, Lactic acid increases ceramide content in human skin. A similar increase is observed in keratinocytes at a concentration of 20 mM after 24 hours, with fold changes ranging from 1.4 to 4.3. It also exhibits cytotoxicity against human MRC5 cells (IC50 of 23,930,000 nM) and A549 cells (IC50 of 26,610,000 nM), determined by the MTT assay after 48 hours..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Lactic acid (2-hydroxypropanoic acid), a glycolysis end product, is a hydroxycarboxylic acid receptor 1 (HCAR1) activator (EC50=4.2-5 mM). Lactic acid can be used as a hemodynamic index in critically ill patients and has anti-tumor activity.
Targets & IC50	GPR109A:1 mM (EC50), GPR81 (HCAR1):1 mM (EC50), GPR109B:1 mM (EC50)
In vitro	METHODS: : Effects on cell proliferation and migration of uveal melanoma after treatment with Lactic acid (20 mM) for 24 and 48 hours. RESULTS: Lactic acid led to a significant reduction in cell proliferation and migration. [1] Method: Uveal melanoma was treated with Lactic acid (20 mM) for 24 hours, and the protein expression level was detected by western blot. Result: Lactic acid promoted the increase in the protein expression levels of MCT1 and HCAR1. [1]
In vivo	METHODS: To detect the anti-tumor activity in vivo, Lactic acid (1.6 g/kg) was subcutaneously injected into Female C57BL/6 mice with MC38 colon cancer cells; pH 7.4 Once a day; From Day 8 to Day 30. RESULTS: Lactic acid demonstrated CD8+ T cell-dependent tumor growth inhibition in the MC38 colon cancer model. [2]
Synonyms	2-hydroxypropanoic acid

Chemical Properties

Molecular Weight	90.08
Formula	C₃H₆O₃
Cas No.	50-21-5
Smiles	O=C(O)C(O)C
Relative Density.	1.31g/cm3

Storage & Solubility Information

Storage

Pure form: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 90 mg/mL (999.11 mM), Sonication is recommended.

H2O: 100 mg/mL (1110.12 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 5 mg/mL (55.51 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO/H2O

	1mg	5mg	10mg	50mg
1 mM	11.1012 mL	55.5062 mL	111.0124 mL	555.0622 mL
5 mM	2.2202 mL	11.1012 mL	22.2025 mL	111.0124 mL
10 mM	1.1101 mL	5.5506 mL	11.1012 mL	55.5062 mL
20 mM	0.5551 mL	2.7753 mL	5.5506 mL	27.7531 mL
50 mM	0.2220 mL	1.1101 mL	2.2202 mL	11.1012 mL
100 mM	0.1110 mL	0.5551 mL	1.1101 mL	5.5506 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

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Keywords

Lactic acid

Related Tags: Lactic acid chemical structure | Lactic acid in vivo | Lactic acid in vitro | Lactic acid formula | Lactic acid molecular weight