Lens culinaris Lectin (LcH)
Referentie L1202-100
Formaat : 100mg
Merk : GLYcoDiag
Lens culinaris lectin (LcH)
| Reference | Short Name | Common Name | Inhibitory Carbohydrate | Glycans Structures Specifities |
|---|---|---|---|---|
| L1202 | LcH | Lens culinaris | MeαMan | Manα/Glcα > GlcNAcα, core GlcNAc of N-linked glycopeptides with a Fuc linked α6. |
Lens culinaris lectin or agglutinin (LcH) is isolated from Lens culinaris (lentil) seeds and purified by affinity chromatography. The lectin which has a molecular weight of 46 kDa (1), is a mix of two isolectins LcH-A and LcH-B of same sugar specificity. Each isolectin is a tetramer composed of two different subunits : the a-subunit (Mr 5710) and the b-subunit (Mr 17 570) (2).
LcH’s carbohydrate specificity is D-mannose and D-glucose enhanced by Fuca6 on the core GlcNAc-Asn N-glycopeptides (3). The detailed specificity of LcH was also assessed by the hemagglutination inhibition assay using various glycopeptides (4) and by X-ray cristallography (5). These studies concluded that LcH recognizes core-fucosylated, bi-antennary N-glycans with terminal galactose residues at the nonreducing ends. LcH agglutinates human red blood cells, although this reaction is not blood group specific. According its specificity, LcH is used for Alpha-fetoprotein detection as this glycoprotein is a useful marker for hepatocellular carcinoma (6, 7).
Applications
Affinity chromatography
Validated in GLYcoPROFILE
Hemagglutination studies
Glycoconjugates separation
Features
Sugar specificity: α-Man, Glc
Haemagglutinating activity
Lyophilized powder
Ordering informations
| Reference | Name | Quantity |
|---|---|---|
| L1202-100 | LcH lectin | 100 mg |
| L1202-25 | LcH lectin | 25 mg |
| L1202-10 | LcH lectin | 10 mg |
References
(1) Howard IK, Sage HJ. (1969). Isolation and characterization of a phytohemagglutinin from the lentil. Biochemistry, 6, 2436-41.
(2) Foriers A., Lebrung E., , Van Rapenbusch R., de Neve R., Strosberg AD (1981).The Structure of the Lentil (Lens culinaris) Lectin Amino acid sequence determination and prediction of the secondary structure. J. Biol. Chem., 286, 5550-5560.
(3) Kornfeld K, Reitman ML, Kornfeld R. (1981). The carbohydrate-binding specificity of pea and lentil lectins. Fucose is an important determinant. J Biol Chem., 256, 6633–6640.
(4) Debray H, Decout D, Strecker G, Spik G, Montreuil J. (1981). Specificity of twelve lectins towards oligosaccharides and glycopeptides related to N-glycosylproteins. Eur. J. Biochem., 117, 41-55.
(5) Casset, F., Hamelryck, T., Loris, R., Brisson JR, Tellier C, Dao-Thi MH, Wyns L, Poortmans F, Pérez S and Imberty A (1995). NMR, molecular modeling, and crystallographic studies of lentil lectin- sucrose interaction. J Biol Chem., 270, 25619-28.
(6) Nakagawa T, Miyoshi E, Yakushijin T, Hiramatsu N, Igura T, Hayashi N, Taniguchi N, Kondo A. (2008). Glycomic analysis of alpha-fetoprotein L3 in hepatoma cell lines and hepatocellular carcinoma patients. J Proteome Res., 7, 2222–2233.
(7) Tateno H., Nakamura-Tsuruta S. and Hirabayashi J. (2009). Comparative analysis of core-fucose-binding lectins from Lens culinaris and Pisum sativum using frontal affinity chromatography. Glycobiology, 19, 527-536.
| Reference | Short Name | Common Name | Inhibitory Carbohydrate | Glycans Structures Specifities | |
|---|---|---|---|---|---|
| L1222 | ABA | Agaricus Bisporus | Gal/GalNAc | Gal(β-1,3) GaINAc | |
| L1221 | AIA / Jacalin | Artocarpus intergrifolia | Gal | Galα1-6 or Galβ1-3GalNAc (T-antigen)>> lactose, more specific for T-antigen than PNA | |
| L1367 | AML | Astragalus membranaceus | Gal | Galβ |
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