LIM kinase 2 (LIMK2) (NM_005569) Human 3' UTR Clone
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SKU
SC215764
3' UTR clone of LIM domain kinase 2 (LIMK2) transcript variant 2a for miRNA target validation
Other products for "LIM kinase 2 or NM_005569"
Specifications
Product Data
Vector
pMirTarget
Species
Human
Transfection Reporter
RFP
Assay Reporter
Luciferase
E. coli Selection
Kanamycin (25 ug/mL)
Mammalian Cell Selection
Neomycin
Target Symbol
LIM kinase 2
ACCN
NM_005569
Insert Size
1669 bp
Sequence Data
Insert Sequence
>SC215764 3’UTR clone of NM_005569 The sequence shown below is from the reference sequence of NM_005569. The complete sequence of this clone may contain minor differences, such as SNPs. Blue=Stop Codon Red=Cloning site
Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Components
The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
Note
Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2008
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
