LUM siRNA (Rat)
Referentie MBS829402-30nmol
Formaat : 30nmol
Merk : MyBiosource
LUM siRNA – Lumican (Rat)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS829402MBS829402 is an siRNA targeting LUM. Also known as LUM siRNA (Rat). Reactive in Rat. Host: Synthetic. siRNA to inhibit LUM expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
LUM, siRNA
Full Product Name
LUM siRNA (Rat)
Product Gene Name
LUM sirna
Product Synonym Names
LCN; LDC; Lumican; Keratan sulfate proteoglycan lumican; KSPG lumican
Host
Synthetic
Species Reactivity
Rat
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
LUM siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
38,279 Da
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat LUM gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for LUM sirna
RNA Interference (RNAi)
Other Notes
Small volumes of LUM sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for LUM. It may not necessarily be applicable to this product.
NCBI Official Full Name
lumican
NCBI Official Synonym Full Names
lumican
NCBI Official Symbol
Lum
UniProt Protein Name
Lumican
UniProt Synonym Protein Names
Keratan sulfate proteoglycan lumican; KSPG lumican
Protein Family
Lumican
UniProt Gene Name
Lum
UniProt Synonym Gene Names
Lcn; Ldc; KSPG lumican
UniProt Entry Name
LUM_RAT
NCBI Protein Information
lumican
NCBI Summary for LUM
member of leucine-rich proteoglycan family; may play a role in immature and transient fibrosis of acute pancreatitis [RGD, Feb 2006]
UniProt Comments for LUM
LUM: a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. [provided by RefSeq, Jul 2008]
Protein type: Secreted, signal peptide; Secreted
Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; fibrillar collagen
Molecular Function: collagen binding
Biological Process: collagen fibril organization; visual perception; cartilage development; positive regulation of transcription from RNA polymerase II promoter; response to organic cyclic substance
Show more Protein type: Secreted, signal peptide; Secreted
Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; fibrillar collagen
Molecular Function: collagen binding
Biological Process: collagen fibril organization; visual perception; cartilage development; positive regulation of transcription from RNA polymerase II promoter; response to organic cyclic substance
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with LUM sirna
| Reference Product | PubMed Publications |
|---|---|
| Dcn sirna | >20 publications with LUM and Dcn |
| Kera sirna | >12 publications with LUM and Kera |
| Fmod sirna | >10 publications with LUM and Fmod |
| Prelp sirna | >3 publications with LUM and Prelp |
| Ogn sirna | >2 publications with LUM and Ogn |
| Omd sirna | >1 publications with LUM and Omd |
Pathways associated with LUM sirna
| Products by Pathway | Pathway Diagram |
|---|---|
| Extracellular Matrix Organization Pathway antibodies | Extracellular Matrix Organization Pathway Diagram |
| Glycosaminoglycan Metabolism Pathway antibodies | Glycosaminoglycan Metabolism Pathway Diagram |
| Integrin Cell Surface Interactions Pathway antibodies | Integrin Cell Surface Interactions Pathway Diagram |
| Keratan Sulfate Biosynthesis Pathway antibodies | Keratan Sulfate Biosynthesis Pathway Diagram |
| Keratan Sulfate Degradation Pathway antibodies | Keratan Sulfate Degradation Pathway Diagram |
| Keratan Sulfate/keratin Metabolism Pathway antibodies | Keratan Sulfate/keratin Metabolism Pathway Diagram |
| Metabolism Pathway antibodies | Metabolism Pathway Diagram |
| Metabolism Of Carbohydrates Pathway antibodies | Metabolism Of Carbohydrates Pathway Diagram |
| Proteoglycans In Cancer Pathway antibodies | Proteoglycans In Cancer Pathway Diagram |
| Proteoglycans In Cancer Pathway antibodies | Proteoglycans In Cancer Pathway Diagram |
Diseases associated with LUM sirna
| Disease Name | Pubmed Publications |
|---|---|
| Nervous System Diseases Antibodies | >251 publications with LUM and Nervous System Diseases |
| Brain Diseases Antibodies | >150 publications with LUM and Brain Diseases |
| Lung Diseases Antibodies | >122 publications with LUM and Lung Diseases |
| Inflammation Antibodies | >80 publications with LUM and Inflammation |
| Disease Models, Animal Antibodies | >72 publications with LUM and Disease Models, Animal |
| Breast Neoplasms Antibodies | >68 publications with LUM and Breast Neoplasms |
| Kidney Diseases Antibodies | >64 publications with LUM and Kidney Diseases |
| Necrosis Antibodies | >54 publications with LUM and Necrosis |
| Liver Diseases Antibodies | >52 publications with LUM and Liver Diseases |
| Adenocarcinoma Antibodies | >48 publications with LUM and Adenocarcinoma |
Organs/Tissues associated with LUM sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Lung Antibodies | >164 publications with LUM and Lung |
| Brain Antibodies | >127 publications with LUM and Brain |
| Kidney Antibodies | >96 publications with LUM and Kidney |
| Heart Antibodies | >85 publications with LUM and Heart |
| Eye Antibodies | >78 publications with LUM and Eye |
| Connective Tissue Antibodies | >42 publications with LUM and Connective Tissue |
| Embryonic Tissue Antibodies | >30 publications with LUM and Embryonic Tissue |
| Prostate Antibodies | >22 publications with LUM and Prostate |
| Adipose Tissue Antibodies | >18 publications with LUM and Adipose Tissue |
| Colon Antibodies | >16 publications with LUM and Colon |