Reagentia en instrumenten voor immunologie, celbiologie en moleculaire biologie.

M344 [251456-60-7]

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Referentie NB-64-11091-1mL

Formaat : 1mLx10mM(inDMSO)

Merk : Neo Biotech

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M344

(Synonyms: MS 344, Histone Deacetylase Inhibitor III) Copy Product Info

Synonyms: MS 344, Histone Deacetylase Inhibitor III

Catalog No. T2157 Copy Product Info

Purity: 98.18%

M344 (Histone Deacetylase Inhibitor III) is a potent HDAC inhibitor with IC₅₀ of 100 nM and able to induce cell differentiation.

Cas No. 251456-60-7

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:98.18%

Color:White

Product Information

Bioactivity

Description	M344 (Histone Deacetylase Inhibitor III) is a potent HDAC inhibitor with IC₅₀ of 100 nM and able to induce cell differentiation.
Targets & IC50	HDAC:100 nM
In vivo	In vitro, M344 showed significant antiproliferative activity against endometrial cancer cell lines Ishikawa and ovarian cancer cell line SK-OV-3 with EC50 of 2.3 μM and 5.1 μM, respectively, whereas normal human endometrial epithelial cells were almost insensitive to M344. M344 was toxic at concentrations higher than 10 μM, and only up to 20% of the surviving cell population was induced to differentiate. In addition, M344 caused a decrease in the proportion of cells in the S-phase and an increase in the proportion of cells in the G0/G1 phase of the cell cycle, induced apoptosis, and decreased the transmembrane potential of mitochondria.
Synonyms	MS 344, Histone Deacetylase Inhibitor III
Kinase Assay	Enzyme Inhibition : Radioactively labeled chicken core histones are used as the enzyme substrate. The enzyme liberated tritiated acetic acid from the substrate which is quantitated by scintillation counting. IC50 values are results of triple determinations. 50 μL of maize enzyme (at 30 °C) is incubated (30 minutes) with 10 μL of total [3H]acetate-prelabeled chicken reticulocyte histones (1 mg/mL). Reaction is stopped by addition of 36 μL of 1 M HCl/0.4 M acetate and 800 μL of ethyl acetate. After centrifugation (10000 g, 5 minutes) an aliquot of 600 μL of the upper phase is counted for radioactivity in 3 mL of liquid scintillation cocktail. M344 is tested in a starting concentration of 40 μM, and active substances are diluted further.
Cell Research	MEL DS19 cells (murine erythroleukemia cells) are maintained in D-MEM containing 100 units/mL penicillin G sodium and 100 μg/mL streptomycin sulfate supplemented with 10% fetal bovine serum at 37 °C in a 5% CO2 atmosphere. To test M344 for potential to induce cell differentiation, log-phase cells with a population doubling time of 11−13 hours are used. Serial dilutions of M344 are prepared in 24-well plates using 1 mL of D-MEM/well. If M344 are dissolved in DMSO, control wells contains the same amount of solvent (generally 2 μL/mL of medium). Subsequently, the cell suspension is added to the wells. After 72 hours the experiment is evaluated. Cell numbers are counted using a Casy 1 TTC flow cytometer. The proliferation of treated cells is expressed as percent proliferation in comparison with the solvent control. Differentiated MEL cells accumulate hemoglobin. Therefore, the induction of cell differentiation is determined by benzidine staining according to the literature. To 100 μL of cell suspension is added 10 μL of a 0.4% solution of benzidine in 12% acetic acid containing 2% Water2. Within 5 minutes hemoglobin-containing cells stains blue. Benzidine-positive and -negative cells are counted under the microscope in a hemocytometer, and the percentage of positive cells is calculated. M344 is first tested at 10 μM and 50 μM final concentration. According to activity/toxicity profile, a range of concentrations is chosen for a dose−response analysis. (Only for Reference)

Chemical Properties

Molecular Weight	307.39
Formula	C₁₆H₂₅N₃O₃
Cas No.	251456-60-7
Smiles	CN(C)c1ccc(cc1)C(=O)NCCCCCCC(=O)NO
Relative Density.	1.137 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Ethanol: 4 mg/mL (13.01 mM), Sonication is recommended.

H2O: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 62 mg/mL (201.7 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (6.51 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	3.2532 mL	16.2660 mL	32.5320 mL	162.6598 mL
5 mM	0.6506 mL	3.2532 mL	6.5064 mL	32.5320 mL
10 mM	0.3253 mL	1.6266 mL	3.2532 mL	16.2660 mL

DMSO

	1mg	5mg	10mg	50mg
20 mM	0.1627 mL	0.8133 mL	1.6266 mL	8.1330 mL
50 mM	0.0651 mL	0.3253 mL	0.6506 mL	3.2532 mL
100 mM	0.0325 mL	0.1627 mL	0.3253 mL	1.6266 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

Calculator

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

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