Disease:

Osteosarcoma

Origin:

From an osteosarcoma of a 14 year old male. Produces high yields of interferon following superinduction with polyinosinic acid-polycytidilic acid, cyclohexamide and actinomycin. Antigenically, MG-63 interferon is more closely related to human fibroblast than to leukocyte interferon.

Species:

Homo sapiens, human

Tissue:

Bone

Morphology:

Fibroblast

Properties:

Adherent

Cytogenic data:

This is a hypotriploid human cell line. The modal chromosome number was 66 occurring in 44% of cells. The rate of cells with higher ploidies was 2.0%. Eighteen to 19 marker chromosomes were common to all cells.

Patient:

Caucasian, Male, 14 years of age

Medium:

AddexBio-formulated EMEM (C0005-01) + 10% FBS

Subculture:

Subculture at a ratio of 1:4 to 1:8 using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37°C.

Freezing Medium:

Complete growth culture medium supplemented with 5% (v/v) DMSO