400823 | MMP13 293 Cell Lysate Clinisciences

Cell Lysate

Unit

0.1 mg

Cat. No.

400823

Description

Antigen standard for matrix metallopeptidase 13 (collagenase 3) (MMP13) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.

Spec Container

Datasheet

Applications

ELISA

Format

This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).

Alternate Names

collagenase 3; matrix metalloproteinase 13 (collagenase 3); MMP-13; matrix metalloproteinase-13; MMP13; CLG3; MANDP1

Accession No.

NP_002418

Application Notes

WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Storage

Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.

Images

For research use only, not for diagnostic or therapeutic procedures.

MMP13 293 Cell Lysate

Referentie 400823

Merk : Abbiotec

Spec Container

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