RayBio® C-Series Mouse Cytokine Antibody Array 5 Kit. Detects 48 Mouse Cytokines. Suitable for all liquid sample types.
Mouse Cytokine Array C5
Mouse Cytokine Array C5
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Mouse |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 48 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
4-1BB (TNFRSF9/CD137)
| 6Ckine (CCL21)
| ACE
| ALK-1
| Amphiregulin
|
Cardiotrophin-1 (CT-1)
| CD27 (TNFRSF7)
| CD27 Ligand (TNFSF7)
| CD36 (SR-B3)
| CD40 Ligand (TNFSF5)
|
Chordin
| CTLA-4 (CD152)
| Decorin
| DKK-1
| E-Cadherin
|
EGF
| Endoglin (CD105)
| Epigen
| Epiregulin
| Galectin-1
|
Gas 1
| Gas 6
| GITR Ligand (TNFSF18)
| Granzyme B
| HAI-1
|
HGF
| IL-1 R4 (ST2)
| IL-11
| IL-17B
| IL-17E (IL-25)
|
IL-17F
| IL-1 RA (IL-1 F3)
| IL-2 R alpha
| IL-20
| IL-21
|
IL-28A (IFN-lambda 2)
| IL-6 R
| JAM-A (CD321/F11R)
| MAdCAM-1
| MFG-E8
|
Neprilysin
| Pentraxin-3 (TSG-14)
| Prolactin
| RAGE
| TACI (TNFRSF13B)
|
TREM-1
| TWEAK (TNFSF12)
| TWEAK R (TNFRSF12)
| ||
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Mouse Cytokine Antibody Array C5 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
citations
Inhibition of Wnt/-catenin signaling ameliorates osteoarthritis in a murine model of experimental osteoarthritis
- Species:
- Mouse
- Sample Type:
- Conditioned media
- Year:
- 2018
- Journal Name:
- JCI insight
Dickkopf-1 promotes hematopoietic regeneration via direct and niche-mediated mechanisms
- Species:
- Mouse
- Sample Type:
- Bone Marrow Aspirate (Bone marrow supernates)
- Year:
- 2017
- Journal Name:
- Nature Medicine
- PubMed ID:
- 27918563
Rapamycin inhibits the secretory phenotype of senescent cells by a Nrf2-independent mechanism
- Species:
- Mouse
- Sample Type:
- Conditioned Media (Mouse embryonic Fibroblasts)
- Year:
- 2017
- Journal Name:
- Aging Cell
- PubMed ID:
- 28371119
Therapy-induced tumour secretomes promote resistance and tumour progression
- Species:
- Mouse
- Sample Type:
- Conditioned Media (Stroma derived cytokines)
- Year:
- 2015
- Journal Name:
- Nature
- PubMed ID:
- 25807485
Divergent Mucosal and Systemic Responses in Children in Response to Acute Otitis Media
- Species:
- Mouse
- Sample Type:
- Nasopharyngeal Fluid (Nasopharyngeal Washes)
- Year:
- 2014
- Journal Name:
- Clinical and Experimental Immunology
Rab27a supports exosome-dependent and -independent mechanisms that modify the tumor microenvironment and can promote tumor progression
- Species:
- Mouse
- Sample Type:
- Conditioned Media (Scr, 4T1 cells)
- Year:
- 2012
- Journal Name:
- Cancer Research
- PubMed ID:
- 22865453
Amyloid Precursor Protein and Proinflammatory Changes Are Regulated in Brain and Adipose Tissue in a Murine Model of High Fat Diet-Induced Obesity
- Species:
- Mouse
- Sample Type:
- Conditioned Media (Peritoneally ellicited macrophages)
- Year:
- 2012
- Journal Name:
- Plos One
- PubMed ID:
- 22276186
Disruption of bone morphogenetic protein receptor 2 (BMPR2) in mammary tumors promotes metastases through cell autonomous and paracrine mediators
- Species:
- Mouse
- Sample Type:
- Tissue Lysate (Mammary tumor lysates)
- Year:
- 2012
- Journal Name:
- PNAS
Thy1 (CD90) regulates the extravasation of leukocytes during inflammation.
- Species:
- Mouse
- Sample Type:
- BAL Fluid
- Year:
- 2011
- PubMed ID:
- 21264853