Mouse Cytokine Array GS4000

Referentie GSM-CAA-4000-1

Formaat : 16SampleKit

Merk : RayBiotech


Mouse Cytokine Array GS4000

G-Series Mouse Cytokine Array 4000 Kit. A combination of 5 non-overlapping arrays to measure the relative expression levels of 200 mouse cytokines. Suitable for all liquid sample types.
Contact Us for Bulk Request

Product Description

Specifications

Size16 Sample Kit, 32 Sample Kit, 64 Sample Kit
SpeciesMouse
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-QuantitativeSemi-Quantitative
Number of Targets Detected200
Solid Support
Glass Slide
Design PrincipleSandwich-based
Method Of DetectionFluorescence Laser Scanner
Shipping TypeBlue ice
Storage-20°C

Introduction

RayBio® G-Series Arrays are glass slide-based antibody arrays which allow researchers to conduct rapid, accurate expression profiling of hundreds of cytokines, chemokines, growth factors, proteases, soluble receptors and other proteins from any biological fluid. Like a traditional sandwich-based ELISA, this array uses a matched pair of cytokine-specific antibodies for detection. Like the Quantibody® Multiplex ELISA Arrays, G-Series utilizes a highly sensitive and stable fluorescent readout which can be detected by most laser fluorescent scanner systems (see below for more info). After capturing the spot densities with a laser scanner, normalization of the raw data can be easily calculated by the researcher, or by a quick copy-paste into our excel-based Analysis Tool software.

This array as well as all catalog numbers beginning with 'GS' differ from the classic G-Series Arrays in a few important ways. First, each capture antibody is printed in quadruplicate instead of duplicate, delivering higher precision. Secondly, this array features the same antibody panels used in our Quantibody Arrays, allowing a seamless transition to our quantitative multiplex assay platform. Lastly, all 16 wells are spotted as sub-arrays, delivering easy handling of 16 samples simultaneously while consuming low sample volumes (10 - 100 µl per array).

Product Features

  • High sensitivity (pg/mL to ng/mL levels)
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Test 16 samples on each slide
  • Data analysis software provides one-step computation

Target Names

Scroll over each target protein for more information
4-1BB
(TNFRSF9/CD137)
6Ckine
(CCL21)
Ace
Activin A
ADAMTS-1
(METH1)
Adiponectin
(ACRP30)
ALK-1
Amphiregulin
ANGPTL1
ANGPTL3
Artemin
Axl
BAFF R
Betacellulin
(BTC)
bFGF
BLC
(CXCL13)
C5a
Cardiotrophin-1
(CT-1)
CCL28
(MEC)
CCL6
CD27
(TNFRSF7)
CD27 Ligand
(TNFSF7)
CD30
(TNFRSF8)
CD30 Ligand
(TNFSF8)
CD32a
CD36
(SR-B3)
CD40
(TNFRSF5)
CD40 Ligand
(TNFSF5)
CD48
(SLAMF2)
CD6
CD80
(B7-1)
Chemerin
Chordin
Clusterin
CRP
(C-Reactive Protein)
CTLA-4
(CD152)
CXCL16
Cystatin C
DAN
Decorin
DKK-1
DLL4
Dtk
E-Cadherin
EDAR
EGF
Endocan
Endoglin
(CD105)
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Epigen
Epiregulin
E-Selectin
Fas
(TNFRSF6/Apo-1)
Fas Ligand
(TNFSF6)
Fetuin A
Flt-3 Ligand
Fractalkine
(CX3CL1)
Galectin-1
Galectin-3
Galectin-7
Gas 1
Gas 6
GCSF
GITR
(TNFRSF18)
GITR Ligand
(TNFSF18)
GM-CSF
gp130
Granzyme B
Gremlin-1
H60
HAI-1
HGF
HGFR
I-309
(TCA-3/CCL1)

Species Detected

Mouse

Application Notes

Suggested Application
  • Multiplexed Protein Detection
  • Detection of Relative Protein Expression
  • Detecting Patterns of Cytokine Expression
  • Biomarker Screening
  • Identifying Key Factors
  • Confirming a Biological Process
Kit Components
  • Antibody Array Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • 30 ml Centrifuge Tube
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

documents

GAL (GAL-QAM-CYT-4.gal)
GAL (GAL-QAM-CYT-5.gal)
GAL (GAL-QAM-CYT-6.gal)
GAL (GAL-QAM-CYT-7.gal)
GAL (GAL-QAM-CYT-8.gal)
Excel (GSM-CYT-4_Analysis_Tool.xlsx)
Excel (GSM-CYT-5_Analysis_Tool.xlsx)
Excel (GSM-CYT-6_Analysis_Tool.xlsx)
Excel (GSM-CYT-7_Analysis_Tool.xlsx)
Excel (GSM-CYT-8_Analysis_Tool.xlsx)

citations

  • Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis

    Species:
    Mouse
    Sample Type:
    Tissue Lysate (Skin samples in psoriasis model)
    Year:
    2020
    Journal Name:
    Immunity
    PubMed ID:
    32553276
  • Sodium oligomannate therapeutically remodels gut microbiota and suppresses gut bacterial amino acids-shaped neuroinflammation to inhibit Alzheimer's disease progression

    Species:
    Mouse
    Sample Type:
    Tissue Lysate (brain tissue lysates from AD and normal patients)
    Year:
    2019
    Journal Name:
    Cell Research
  • Fate mapping RNA-sequencing reveal Malat1 regulates Sca1+ progenitor cells to vascular smooth muscle cells transition in vascular remodeling

    Species:
    Mouse
    Sample Type:
    Sca1+ stem/progenitor cells (SPCs)
    Year:
    2023
    Journal Name:
    Cellular and Molecular Life Sciences
  • Inducing the “re-development state” of periodontal ligament cells via tuning macrophage mediated immune microenvironment

    Species:
    Mouse
    Sample Type:
    RAW264.7 cell
    Year:
    2023
    Journal Name:
    Journal of advanced research
  • Injury-induced interleukin-1 alpha promotes Lgr5 hair follicle stem cells de novo regeneration and proliferation via regulating regenerative microenvironment in mice

    Species:
    Mouse
    Sample Type:
    Dorsal skin tissues
    Year:
    2023
    Journal Name:
    Inflammation and regeneration
  • Progesterone prevents HGSOC by promoting precancerous cell pyroptosis via inducing fibroblast paracrine

    Species:
    Mouse
    Sample Type:
    ascites
    Year:
    2023
    Journal Name:
    iScience
  • Single-Cell Sequencing Informs That Mesenchymal Stem Cell Alleviates Renal Injury Through Regulating Kidney Regional Immunity in Lupus Nephritis

    Species:
    Mouse
    Sample Type:
    kidney tissue
    Year:
    2023
    Journal Name:
    Stem Cells and Development
  • Correction: A novel lymphatic pattern promotes metastasis of cervical cancer in a hypoxic tumour-associated macrophage-dependent manner

    Species:
    Human
    Sample Type:
    THP-1 cell, Human Dermal Lymphatic Endothelial Cells (HDLEC)
    Year:
    2022
    Journal Name:
    Angiogenesis
  • ORFV infection enhances CXCL16 secretion and causes oncolysis of lung cancer cells through immunogenic apoptosis

    Species:
    Mouse
    Sample Type:
    LLC cell-xenograft, A549 cell-xenograft
    Year:
    2022
    Journal Name:
    Frontiers in cellular and infection microbiology
  • Minmin Jiang, Wenying Cai, Junmin Zhang, Ting Xie, Liyan Xi, Xiqing Li, Jiufeng Sun, Melanization of a meristematic mutant of Fonsecaea monophora increase the pathogenesis in a BALB/c mice infection model, Medical Mycology, Volume 56, Issue 8, November 2018, Pages 979-986, https://doi.org/10.1093/mmy/myx148

    Species:
    Mouse
    Sample Type:
    Serum
    Year:
    2018
    Journal Name:
    Medical Mycology

Misschien heeft u ook interesse in de volgende producten:



Referentie
Beschrijving
Cond.
Price Bef. VAT
QAM-CAA-4000-1
 8SampleKit 
AAM-INF-1-2
 2SampleKit