Mouse Tartrate-Resistant Acid Phosphatase 5 / TRACP5 (ACP5) ELISA Kit is an ELISA Kit for the in vitro quantitative measurement of Mouse Tartrate-Resistant Acid Phosphatase 5 / TRACP5 (ACP5) concentrations in serum, plasma, tissue homogenates and other biological fluids.

Introduction	Tartrate-resistant acid phosphatase (TRAP or TRAPase), also called acid phosphatase 5, tartrate resistant (ACP5), is a glycosylated monomeric metalloprotein enzyme expressed in mammals. It has a molecular weight of approximately 35 kDa, a basic isoelectric point (7.6–9.5), and optimal activity in acidic conditions. TRAP is synthesized as latent proenzyme and activated by proteolytic cleavage and reduction. It is differentiated from other mammalian acid phosphatases by its resistance to inhibition by tartrate and by its molecular weight. TRAP is associated with osteoclast migration to bone resorption sites, and, once there, TRAP is believed to initiate osteoclast differentiation, activation, and proliferation.
Target	Tartrate-Resistant Acid Phosphatase 5 / TRACP5 (ACP5)
Reactivity	Mouse
Tested Applications	ELISA
Recommended dilutions	Optimal dilutions/concentrations should be determined by the end user.
Storage	Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
Validity	The validity for this kit is at least 6 months. Up to 12 months validity can be provided on request.
Stability	The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.
Test Range	0.13 ng/ml - 8 ng/ml
Sensitivity	0.08 ng/ml
Standard Form	Lyophilized
Detection Method	Colorimetric
Assay Type	Sandwich
Assay Data	Quantitative
Sample Type	Serum, plasma, tissue homogenates and other biological fluids.
Kit Components	The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product. Pre-coated 96-Well Microplate Standard Standard Diluent Buffer Wash Buffer Detection Reagent A Detection Reagent B Diluent A Diluent B TMB Substrate Stop Solution Plate Sealer
Material Required But Not Provided	37°C incubator Multi and single channel pipettes and sterile pipette tips Squirt bottle or automated microplate washer 1.5 ml tubes Distilled water Absorbent filter papers 100 ml and 1 liter graduated cylinders Microplate reader (wavelength: 450 nm) ELISA Shaker
Assay Procedure	This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product. 1) Set standard, test samples and control wells. 2) Aliquot 100 µl of diluted standard into the standard wells. 3) Aliquot 100 µl of Standard Diluent buffer into control (zero) well. 4) Aliquot 100 µl of diluted samples into the sample wells. Incubate for 1 hr at 37 °C. 5) Aliquot 100 µl of Detection Reagent A to each well. Incubate for 1 hr at 37 °C. 6) Wash 3 times. 7) Aliquot 100 µl of Detection Reagent B to each well. Incubate for 90 mins at 37 °C. 8) Wash 5 times. 9) Aliquot 90 µl of TMB Substrate to each well. Incubate for 10-20 mins at 37 °C. 10) Aliquot 50 µl of Stop Solution. 11) Measure the OD at 450 nm.
Assay Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Tartrate-Resistant Acid Phosphatase 5 / TRACP5 (ACP5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Tartrate-Resistant Acid Phosphatase 5 / TRACP5 (ACP5) were tested on 3 different plates, 8 replicates in each plate. CV (%) = (Standard Deviation / mean) × 100 Intra-Assay: CV<10% Inter-Assay: CV<10%
Availability	Shipped within 5-7 working days.
Note	This product is for research use only. The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments. Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.
Plate coated with	Antibody

Research Articles on Tartrate-Resistant Acid Phosphatase 5 / TRACP5 (ACP5)