Murashige and Skoog (MS) Basal Medium is the most popular culture medium in modern plant tissue culture research. It is a complex growth medium with all of the micronutrients and macronutrients needed for plants to thrive. MS Medium is effective on an endless list of plant species.

Murashige and Skoog Basal Medium should be dissolved at 4.33 g/L of distilled water and autoclave. Cytokines, Auxins, Vitamins, and Sucrose may be added as needed. Phytoagar may be added at 8-15 g/L before autoclaving for use as a solid agar growth medium.

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References

Murashige & Skoog (MS) Medium contains Macronutrients and Micronutrients as described by Murashige & Skoog (1967).

See "Murashige T & Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures" by the University of Pennsylvania.