Murine Interleukin-3 (rMuIL-3 )
Referentie orb1495010-1mg
Formaat : 1.0mg
Merk : Biorbyt
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Murine Interleukin-3 (rMuIL-3 )
Catalog Number: orb1495010
| Catalog Number | orb1495010 |
|---|---|
| Category | Proteins |
| Description | IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoieses, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is species-specific, variably glycosylated cytokine. |
| Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| MW | Approximately 15.1 kDa globular protein containing 135 amino acid residues. |
| Protein Sequence | MDTHRLTRTL NCSSIVKEII GKLPEPELKT DDEGPSLRNK SFRRVNLSKF VESQGEVDPE DRYVIKSNLQ KLNCCLPTSA NDSALPGVFI RDLDDFRKKL RFYMVHLNDL ETVLTSRPPQ PASGSVSPNR GTVEC |
| Source | Escherichia coli. |
| Biological Activity | The ED50 as determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is 2 x 107 units/mg. |
| Endotoxins | Less than 1EU/mg of rmIL-3 as determined by LAL method. |
| Storage | This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles. |
| Buffer/Preservatives | Lyophilized from a 0.2mm filtered solution in PBS, pH 7.4. |
| Note | For research use only |
| Application notes | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Expiration Date | 6 months from date of receipt. |