OKEH02623 | Oxytocin ELISA Kit Clinisciences

Technical Documents

Datasheets/Manuals	Printable datasheet for Oxytocin ELISA Kit (OKEH02623)
COA Datasheet	Printable COA datasheet for OKEH02623

Product Info

Predicted Species Reactivity

All Species

Application

ELISA-Comp

ELISA Kit Detection Method

Colorimetric, OD 450 nm

ELISA Kit Duration

~ 3 Hours

ELISA Kit Principle

Aviva Systems Biology Oxytocin ELISA Kit (OKEH02623) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Oxytocin antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Oxytocin and incubated. The Oxytocin found in the sample or standards competes with the biotinylated Oxytocin for limited binding sites on the immobilized anti-Oxytocin antibody. Excess unbound biotinylated Oxytocin and sample or standard Oxytocin is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Oxytocin captured in the well and inversely proportional to the amount of Oxytocin which was contained in the sample or standard.

ELISA Kit Range

31.2-2000pg/mL

ELISA Kit Recovery

Mean recovery when spiking into Serum and Plasma = 93%

ELISA Kit Reproducibility

Mean Intra-assay CV%: < 4.8% (n = 20)
Mean Inter-assay CV%: < 7.4% (n = 20)

ELISA Kit Component

Component	Amount
Anti-Oxytocin Microplate	96 Wells (12 x 8 Well strips)
Oxytocin Lyophilized Standard	2 x 1,000 pg
Sample Diluent	1 x 20 mL
100X Oxytocin-Biotin Complex	1 x 60 uL
100X Avidin-HRP Conjugate	1 x 120 uL
Biotin Complex Diluent	1 x 12 mL
Conjugate Diluent	1 x 12 mL
25X Wash Buffer	1 x 30 mL
TMB Substrate	1 x 10 mL
Stop Solution	1 x 10 mL

Additional Information

Target Abbr: Oxtc
Target Name: Oxytocin

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Pubchem: 439302

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Chemical formula: C₄₃H₆₆N₁₂O₁₂S₂

Reconstitution and Storage

Store as indicated in product manual.

Sample Type

Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids.

Sensitivity

9.4 pg/mL

Specificity

Natural and recombinant General Oxytocin

Assay Info

Assay Methodology: Quantitative Competitive ELISA

Target Info

Alias Symbols	Oxtc, Oxytocin, Oxtc
Protein Name	Oxytocin
Description of Target	Recombinant Oxytocin is a synthetic cyclic peptide form of the naturally occurring posterior pituitary hormone oxytocin. Oxytocin binds to oxytocin receptors in the uterine myometrium, which triggers the G-protein coupled receptor signal transduction cascade that causes increased intracellular calcium concentrations. Increased calcium concentration levels activate myosin light chain kinase which, in turn, induces the formation of the contractile protein actomyosin. This stimulates uterine smooth muscle contractions. This agent also stimulates smooth muscles in the mammary glands, thereby causing lactation.

Protocols & Data

Oxytocin ELISA Kit

Referentie OKEH02623

Merk : Aviva Systems Biology

Oxytocin ELISA Kit (OKEH02623)

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