This buffer solution should be stored between 2°C and 8°C.

Stable for 12 months from the date of manufacture.

Permeabilization Wash Buffer (10X) is used for preparing cells for staining intracellular cytokines or other proteins. Permeabilization Wash Buffer (10X) is used to permeabilize cells following fixation with 10X Fixative Buffer (Leinco Product No.: F1194). Supplied as a 10X solution, deionized water should be used to dilute the solution before use. Permeabilization Wash Buffer (10X) is formulated to reduce non-specific staining, enhance the signal to noise ratio, and has minimal effects of cell. Useful for antibody dilutions and cell washing during intracellular staining.

To permeabilize cells, it is recommended to first dilute Permeabilization Wash Buffer (10X) to 1X solution using deionized water. After dilution of Permeabilization Wash Buffer (10X), resuspend cells and centrifuge, repeat this process twice. Titration should occur before each application.