Phosphoglucomutase 5 (PGM5) (NM_021965) Human Tagged ORF Clone
CAT#: RG218774
- TrueORF®
PGM5 (tGFP-tagged) - Human phosphoglucomutase 5 (PGM5)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
Product Images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Symbol | Phosphoglucomutase 5 |
| Synonyms | PGMRP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | >RG218774 representing NM_021965 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGGAGCCCCATCCCGGTGCTGACAGTGCCCACCGCGCCCTACGAGGACCAGCGGCCGGCCGGCG GCGGGGGTCTGCGGCGACCCACCGGCCTCTTCGAGGGCCAGCGCAACTACCTGCCCAACTTTATCCAGAG CGTGCTGTCGTCCATCGACCTGCGCGACCGTCAGGGCTGCACCATGGTGGTGGGCAGCGACGGCAGGTAC TTTAGCAGGACGGCCATCGAGATCGTGGTGCAGATGGCCGCGGCCAACGGGATTGGACGACTGATTATTG GACAGAATGGCATCTTGTCGACACCTGCGGTCTCCTGCATTATCAGGAAGATCAAGGCAGCTGGTGGAAT CATTCTAACAGCCAGCCACTGCCCTGGAGGACCAGGGGGAGAGTTTGGAGTGAAGTTTAATGTTGCCAAT GGAGGTCCTGCACCCGATGTTGTCTCAGACAAAATCTACCAAATCAGCAAAACGATTGAGGAATATGCTA TATGTCCTGATCTCCGAATCGACCTATCTCGACTAGGAAGACAAGAATTTGACCTAGAAAACAAATTCAA ACCATTCAGAGTGGAGATAGTGGACCCAGTGGATATCTATCTTAACCTCCTTCGGACCATCTTTGACTTT CATGCCATCAAGGGTTTGCTGACTGGACCCAGCCAACTGAAGATTCGCATTGACGCAATGCACGGAGTTA TGGGACCTTATGTGAGAAAAGTTCTGTGTGATGAGCTGGGGGCCCCAGCCAATTCTGCAATAAACTGTGT TCCCCTGGAAGACTTTGGAGGGCAGCACCCTGACCCAAACCTGACATATGCAACGACTCTTCTGGAAGCA ATGAAAGGAGGAGAATATGGATTTGGAGCTGCATTTGATGCTGATGGGGACCGTTATATGATCCTAGGCC AAAATGGCTTCTTTGTGAGCCCTTCTGACTCCCTGGCCATCATTGCTGCCAACCTCTCTTGCATTCCATA TTTCCGTCAGATGGGGGTCCGCGGGTTTGGGAGGAGTATGCCAACCAGCATGGCCCTGGACAGAGTGGCC AAATCAATGAAGGTCCCTGTATATGAGACCCCAGCTGGATGGAGATTCTTCTCAAATCTGATGGACTCAG GACGTTGCAATCTGTGTGGGGAAGAGAGCTTTGGCACTGGCTCTGACCACCTCCGAGAGAAGGATGGCCT GTGGGCTGTCTTGGTCTGGCTCTCCATTATTGCTGCCCGGAAGCAGAGTGTGGAGGAAATTGTCCGAGAT CACTGGGCCAAATTTGGCCGCCACTACTATTGCAGGTTTGACTATGAGGGGTTGGATCCCAAGACGACAT ATTATATCATGAGGGACCTGGAGGCCCTGGTCACAGACAAATCCTTCATTGGCCAGCAGTTTGCTGTGGG GAGCCATGTCTACAGCGTGGCGAAGACGGATAGTTTTGAATACGTGGACCCTGTGGATGGCACTGTGACC AAGAAACAGGGCCTAAGGATCATTTTCTCGGATGCATCACGGCTCATCTTCCGGCTCAGTTCCTCCAGTG GTGTGCGGGCCACCCTCAGACTGTACGCAGAGAGCTACGAGAGGGATCCCAGCGGCCATGACCAGGAGCC ACAGGCAGTGCTGAGCCCTCTCATAGCCATCGCACTGAAAATATCCCAGATTCATGAGAGAACTGGCCGG AGGGGACCCACTGTCATCACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG218774 representing NM_021965 Red=Cloning site Green=Tags(s) MEGSPIPVLTVPTAPYEDQRPAGGGGLRRPTGLFEGQRNYLPNFIQSVLSSIDLRDRQGCTMVVGSDGRY FSRTAIEIVVQMAAANGIGRLIIGQNGILSTPAVSCIIRKIKAAGGIILTASHCPGGPGGEFGVKFNVAN GGPAPDVVSDKIYQISKTIEEYAICPDLRIDLSRLGRQEFDLENKFKPFRVEIVDPVDIYLNLLRTIFDF HAIKGLLTGPSQLKIRIDAMHGVMGPYVRKVLCDELGAPANSAINCVPLEDFGGQHPDPNLTYATTLLEA MKGGEYGFGAAFDADGDRYMILGQNGFFVSPSDSLAIIAANLSCIPYFRQMGVRGFGRSMPTSMALDRVA KSMKVPVYETPAGWRFFSNLMDSGRCNLCGEESFGTGSDHLREKDGLWAVLVWLSIIAARKQSVEEIVRD HWAKFGRHYYCRFDYEGLDPKTTYYIMRDLEALVTDKSFIGQQFAVGSHVYSVAKTDSFEYVDPVDGTVT KKQGLRIIFSDASRLIFRLSSSSGVRATLRLYAESYERDPSGHDQEPQAVLSPLIAIALKISQIHERTGR RGPTVIT TRTRPLE - GFP Tag - V |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
| ACCN | NM_021965 |
| ORF Size | 1701 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at info@clinisciences.com The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021965.4 |
| RefSeq Size | 3341 bp |
| RefSeq ORF | 1704 bp |
| Locus ID | 5239 |
| UniProt ID | Q15124 |
| Cytogenetics | 9q21.11 |
| Protein Families | Druggable Genome |
| Gene Summary | Phosphoglucomutases (EC 5.2.2.2.), such as PGM5, are phosphotransferases involved in interconversion of glucose-1-phosphate and glucose-6-phosphate. PGM activity is essential in formation of carbohydrates from glucose-6-phosphate and in formation of glucose-6-phosphate from galactose and glycogen (Edwards et al., 1995 [PubMed 8586438]).[supplied by OMIM, Mar 2008] |
Documents
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Resources
Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| RC218774 | PGM5 (Myc-DDK-tagged)-Human phosphoglucomutase 5 (PGM5) | 791,00 EUR | |
| RC218774L3 | Lenti-ORF clone of PGM5 (Myc-DDK-tagged)-Human phosphoglucomutase 5 (PGM5) | 1 091,00 EUR | |
| RC218774L4 | Lenti-ORF clone of PGM5 (mGFP-tagged)-Human phosphoglucomutase 5 (PGM5) | 1 091,00 EUR | |
| SC304977 | PGM5 (untagged)-Human phosphoglucomutase 5 (PGM5) | 846,00 EUR |