PI 3 Kinase p85 alpha (PIK3R1) Mouse Monoclonal Antibody [Clone ID: AB6]

Specifications

Product Data
Clone Name	AB6
Applications	IF, IP, WB
Recommended Dilution	Western blot: 1 µg/mL for chemiluminescence detection system. Immunoprecipitation: 1-5 µg/600 µl of cell extract from 5x106 cells. Immunocytochemistry:  10 µg/ml.
Reactivities	Human, Mouse, Rat
Host	Mouse
Isotype	IgG1
Clonality	Monoclonal
Immunogen	Recombinant human p85alpha.
Specificity	This antibody reacts with p85alpha, which is regulatory subunit of PI3-kinase on Western blot, Immunoprecipitation and Immunocytochemistry. This antibody recognizes mutant p85alpha protein expressed in E. coli to be possessed in amino acid position '1-79' described below. This antibody does not react with p85beta.
Formulation	PBS containing 50% glycerol, pH 7.2 State: Azide Free State: Liquid Ig fraction without preservatives
Concentration	lot specific
Purification	Protein A agarose
Conjugation	Unconjugated
Storage	Store (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Gene Name	phosphoinositide-3-kinase regulatory subunit 1
Database Link	Entrez Gene 5295 Human UniProt ID P27986
Background	Phosphoinositide 3-kinase (PI3K) family of enzymes generate 3-phosphorylated phosphoinositides that act as second messengers downstream of numerous cellular receptors. This family control several cellular responses including cell growth, survival cytoskeletal remodeling and the trafficking of intracellular organelles in many different cell types. There are four subgroups (class IA, IB, II, III) of PI3K with distinct structure and substrate specificity. Class IA PI3K, which function downstream of activated tyrosine kinases, are heterodimers composed of a p110 catalytic subunit (a, b, or o) and a tightly associated regulatory subunit (p85a, p55a, p50a, p85B, or p55y). Each regulatory subunit can interchangeably associate with different catalytic subunits and shows a unique tissue distribution. p85a is the major regulatory subunit of class IA PI3Ks in most types of cells including immune cells.
Synonyms	PI3K regulatory subunit alpha, PI3-kinase subunit p85-alpha
Note	This product was originally produced by MBL International.
Reference Data