This Lenti vector is a third generation lentiviral vector.

• All OriGene's TrueORF clones can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
• The ORF (no stop codon for P2A-Puro) cloned in this vector will be expressed as native protein, no tag.
• P2A-Puro is within the Lentiviral packaging cassette and would allow puromycin selection in both the transfected and the transduced cells.
• A brief lentiviral packaging protocol is provided.
• E. Coli. selection: Chloramphenicol (34 ug/ml)
• V2_F Primer can be used to sequence the target sequence after cloning.
• P2A-DDK_R Primer can be used to sequence the target sequence after cloning.