Pluronic F-127

Biological Activity

Pluronic F-127 was used for fluorescent labeling of blood vessels, astrocytes, and neurons. Pluronic F-127 is frequently used with dye AM esters such as Indo-1 AM, Fura-2 AM, Calcein AM, Fluo-3 AM, Fluo-4 AM, etc. to improve their water solubility. Pluronic F-127 was used to coat a siliconized coverslip to hold an egg extract in a study. Pluronic F-127 was added to phosphate buffered saline (PBS), to lower unspecific cell and protein adhesion to a PDMS-based microfluidic device.

Chemical Information

CAS Number	9003-11-6
Solubility (25°C)	DMSO 200 mg/mL Water >110 mg/mL (May need warm to 40-50°C)
Storage	2-8°C, protect from light

Conversion of different model animals based on BSA (PMID: 27057123)

Species	Mouse	Rat	Rabbit	Guinea pig	Hamster	Dog
Weight (kg)	0.02	0.15	1.8	0.4	0.08	10
Body Surface Area (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km factor	3	6	12	8	5	20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by	Animal B Km
	Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the K_m factor for a mouse and then divide by the K_m factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Filipe E Antunes, et al. Colloids Surf B Biointerfaces. Gels of Pluronic F127 and nonionic surfactants from rheological characterization to controlled drug permeation