PRMT8, Chemiluminescent BioAssay™ Kit (Protein Arginine N-methyltransferase 8, Histone-arginine N-methyltransferase 8, HRMT1L3, HRMT1L4)

Intended Use:
Suitable for studying enzyme kinetics and HTS applications.

Test Principle:
The PRMT8 Chemiluminescent BioAssay™ Kit is designed to measure PRMT8 activity for screening and profiling applications. The PRMT8 Chemiluminescent BioAssay™ Kit comes in a convenient format with a 96-well plate precoated with histone H4 peptide substrate, the antibody against methylated arginine 3 (R3) residue of Histone H4, the secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified PRMT8 enzyme for 100 enzyme reactions. The key to the PRMT8 Chemiluminescent BioAssay™ Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S- adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Contraindications:
DMSO >1%, strong acids or bases, ionic detergents, high salt

Kit Components:
PRMT8 His-FLAG-tags, 1x10ug
20uM S-adenosylmethionine*, 1x250ul
Primary antibody 4, 1x100ul
Secondary HRP-labeled antibody 2, 1x10ul
4x HMT assay buffer 2, 1x3ml
Blocking buffer, 1x50ml
HRP chemiluminescent substrate A (transparent bottle), 1x6ml
HRP chemiluminescent substrate B (brown bottle), 1x6ml
96-well plate precoated with histone substrate, 1x plate
* Decreasing the S-adenosylmethionine concentration will make the assay more sensitive to test inhibitors.

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.