Propidium Iodide [25535-16-4]
Referentie T2130-25mg
Formaat : 25mg
Merk : TargetMol
Propidium Iodide (Synonyms: PI)
Propidium Iodide
Copy Product InfoSynonyms PI
Cas No. 25535-16-4
For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:99.23%
Color:Red to Purple
COA HNMR HPLC
Product Introduction
Propidium Iodide AI Summary
Propidium Iodide exhibits a diverse range of bioactivities. It shows strong binding affinity for DNA at pH 7, with a measured binding constant greater than 5.0 M^-1. In relation to acetylcholinesterase (AChE), Propidium Iodide has a dissociation constant (Ki) of 1100.0 nM in mouse and inhibits human AChE with an IC50 value of 32.3 µM. Additionally, it demonstrates inhibitory activity against human butyrylcholinesterase (BChE) with an IC50 value of 13.2 µM. The compound also demonstrates an extensive capability to inhibit amyloid beta aggregation; it inhibits human recombinant AChE-induced amyloid beta (1-40) aggregation by 82% at 100 µM, and self-induced amyloid beta (1-40) aggregation by 61.1% at 10 µM. Furthermore, Propidium Iodide exhibits moderate binding affinity for the NMDA receptor and affects calcium signaling pathways. In the context of antiviral activity, the compound inhibits SARS-CoV-2 induced cytotoxicity in Caco-2 cells by 15.98% at a concentration of 10 µM, and shows significant inhibition (76.32%) of the SARS-CoV-2 3CL-Pro protease at 20 µM. Overall, Propidium Iodide demonstrates potential therapeutic implications in neurodegenerative diseases like Alzheimer's and as an antiviral agent against SARS-CoV-2..
Note: Summary generated by AI. Data source: ChEMBL 
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | Propidium Iodide (PI) is a red fluorescent dye utilized for cell staining and is suitable for fluorescence microscopy, confocal microscopy, flow cytometry, and fluorometer analysis. In aqueous solution, the Ex/Em of PI is 493/636 nm. Upon binding with nucleic acid, the Ex/Em shifts to 535/617 nm, enhancing the fluorescence signal 20-30 times. |
| Targets&IC50 | HEK293 cells:1.1 μM |
| In vitro | METHODS: Propidium Iodide uptake and Flow cytometry to detect cell death: 1. Store the PI stock solution (0.5 mg/mL in PBS) in a dark place at 4℃. Immediately before use, prepare PI-FACS buffer by adding 20 µL of PI stock solution per 1 mL of PBS. 2. Collect suspended cells: Collect cells directly in centrifuge tubes and centrifuge at 500g for 5 min to harvest all cells. 3. Collect adherent cells: Remove and preserve the medium containing dead and mitotic cells. Isolate live cells using standard tissue culture techniques, such as incubation with trypsin-EDTA, and be sure to collect any washings (e.g., PBS). Add cells from the culture medium and cells from any wash solution to the isolated cells and centrifuge at 500 g for 5 min to harvest all cells. 4. Resuspend the harvested cells in PI-FACS buffer. The cells were incubated in the dark for 15 min at room temperature. 5. Determine the cell mortality rate by flow cytometry. [1] The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| Cell Research | Propidium Iodide absorption and flow cytometry assay for cell death 1. Store PI stock solution (0.5 mg/mL in PBS) at 4°C in the dark. Immediately before use, prepare PI-FACS buffer by adding 20 µL of PI stock solution to every 1 mL of PBS. 2. Collect suspended cells: Collect cells directly in a centrifuge tube and harvest all cells by centrifugation at 500g for 5 min. 3. Collect adherent cells: Remove and save the culture medium containing dead and mitotic cells. Isolate live cells using standard tissue culture techniques, such as incubation with trypsin-EDTA, and be sure to collect any wash solution (such as PBS). Add cells in the culture medium and cells from any wash solution to the isolated cells and harvest all cells by centrifugation at 500g for 5 min. 4. Resuspend the harvested cells in PI-FACS buffer. Incubate the cells in the dark at room temperature for 15 min. 5. Determine cell death rate by flow cytometry. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| Synonyms | PI |
| Molecular Weight | 668.39 |
| Formula | C27H34I2N4 |
| Cas No. | 25535-16-4 |
| Smiles | [I-].[I-].CC[N+](C)(CC)CCC[n+]1c(-c2ccccc2)c2cc(N)ccc2c2ccc(N)cc12 |
| Relative Density. | 1.5072 g/cm3 (Estimated) |
| Storage | Shipping with blue ice/Shipping at ambient temperature. | ||||||||||||||||||||||||||||||||||||||||
| Solubility Information | H2O: 5 mg/mL (7.48 mM), Sonication and heating to 60℃ are recommended. DMSO: 126.3 mg/mL (188.96 mM), Sonication is recommended.  | ||||||||||||||||||||||||||||||||||||||||
| In Vivo Formulation | 10% DMSO+40% PEG300+5% Tween-80+45% Saline: 3.3 mg/mL (4.94 mM), Sonication is recommended. Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions. | ||||||||||||||||||||||||||||||||||||||||
Solution Preparation Table | |||||||||||||||||||||||||||||||||||||||||
H2O/DMSO
DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | |||||||||||||||||||||||||||||||||||||||||