Rabbit IgG (H&L) Antibody Peroxidase Conjugated
Specifications
Product Data | |
Applications | ELISA, IHC, WB |
Recommended Dilution | WB: 1:1,000 - 1:3,000 IHC: 1:500 - 1:2,000 ELISA: 1:40,000 |
Reactivities | Rabbit |
Host | Donkey |
Clonality | Polyclonal |
Immunogen | Anti-Rabbit IgG (H&L) was produced by repeated immunization with rabbit whole IgG molecule in donkey. |
Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Rabbit IgG and Rabbit Serum. |
Formulation | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL |
Concentration | 2.0 mg/mL - lot specific |
Conjugation | HRP |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Note | Anti-Rabbit IgG peroxidase conjugated antibody has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. |
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