Rat IgG (H+L) Antibody : HRP
Referentie OASB02090
Formaat : 1.0mL
Merk : Aviva Systems Biology
Rabbit Anti-Rat IgG(H+L) Antibody - HRP Conjugated (OASB02090)
| Datasheets/Manuals | Printable datasheet for Rabbit Anti-Rat IgG(H+L) Antibody - HRP Conjugated (OASB02090) |
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| Tested Species Reactivity | Rat |
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| Predicted Species Reactivity | Rat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Conjugation | HRP |
| Application | IHC-F, IHC-P, ICC, WB |
| Additional Information | Description: Pepsin digest of Rabbit Anti-Rat IgG(H+L) - Pooled antisera from rabbits hyperimmunized with rat IgG; purified by affinity chromatography on rat IgG covalently linked to agarose; reacts with the heavy and light chains of rat IgG and the light chains of rat IgM |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Rat IgG |
| Purification | Affinity chromatography on rat IgG covalently coupled to agarose. Gel filtrationchromatography of pepsin digested antibody. |
| Concentration | Lot specific |
| Specificity | IgG(H+L) |
| Characterization | To ensure lot- to- lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flowcytometry for conformance to characteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Immunofluorescence: ELISA: FITC and TRITC conjugates AP conjugate HRP conjugate <= 1 ug/106 cells 1:2,000-1:4,000 1:4,000-1:8,000 |
| Application Info | Direct immunofluorescent staining, Enzyme-Linked-Immunosorbent-Assay (ELISA), Western blotting, Dot-and slot-immunoblotting, Immunohistochemistry |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) and rhodamine (TRITC) conjugates are supplied as 0.5 mg in 1.0 mL PBS/NaN3 Store at 2- 8 C - The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C - Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed. |
| Cross Absorption | NA |
| Gene Full Name | Ig gamma-1 chain C region |
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