Rat IgG2a Antibody : AP
Referentie OASB00771
Formaat : 1.0mL
Merk : Aviva Systems Biology
Rat IgG2a Antibody : AP (OASB00771)
| Datasheets/Manuals | Printable datasheet for Rat IgG2a Antibody : AP (OASB00771) |
|---|
| Tested Species Reactivity | Rat |
|---|---|
| Predicted Species Reactivity | Rat |
| Clonality | Monoclonal |
| Clone | 2A8F4 |
| Isotype | IgG1 |
| Host | Mouse |
| Conjugation | AP |
| Application | IHC-F, ELISPOT |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Rat IgG2a hybridoma |
| Concentration | Lot specific |
| Specificity | IgG2a |
| Characterization | To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested as a second step reagent byflow cytometry and/or ELISA to conform to characteristics of a standard reference reagent. Representativedata are included in this product insert. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eyeor face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact aphysician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds inrunning water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: ELISA: Fluorescein conjugate Biotin conjugate R-phycoerythrin conjugate Cyanine 5 conjugate Horseradish Peroxidase conjugate Alkaline Phosphatase <=1 ug/106 cells <=1 ug/106 cells <=0.1 ug/106 cells <=0.1 ug/106 cells 1:4,000-1:8,000 1:2,000-1:4,000 |
| Application Info | Identification and enumeration of IgG2a+ cells by immunofluorescence microscopy, Second step reagent for rat IgG2a monoclonal antibodies, Enzyme-Linked-Immunosorbent-Assay (ELISA) |
| Other Applications Data | Since applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specificneeds. |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50%Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Storeat 2- 8 C. Do not freeze! - The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored asdirected. |
