Rat IgM Antibody : AP
Referentie OASB01908
Formaat : 1.0mL
Merk : Aviva Systems Biology
Goat Anti-Rat IgM Antibody - AP Conjugated (OASB01908)
| Datasheets/Manuals | Printable datasheet for Goat Anti-Rat IgM Antibody - AP Conjugated (OASB01908) |
|---|
| Tested Species Reactivity | Rat |
|---|---|
| Predicted Species Reactivity | Rat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Conjugation | AP |
| Application | IHC-F, IHC-P, ICC, WB |
| Additional Information | Description: Pooled antisera from goats hyperimmunized with rat IgM; purified by affinity chromatography on pooled rat IgM covalently linked to agarose; reacts with the heavy chain of rat IgM; cross-adsorbed against rat IgG for minimal reactivity |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Rat IgM |
| Purification | Affinity chromatography on rat IgM covalently linked to agarose |
| Concentration | Lot specific |
| Specificity | IgM |
| Characterization | To ensure lot- to- lot consistency, each batch of product is tested by ELISA or FLISA for conformance tocharacteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | FLISA: ELISA: FITC conjugate TRITC and TXRD conjugates R-PE conjugate AP conjugate HRP conjugate BIOT conjugate 1:200 – 1:400 1:100 – 1:400 1ug/mL 1:2,000-1:4,000 1:4,000-1:8,000 1:5,000-1:20,000 |
| Application Info | Direct immunofluorescent staining of IgM+ rat B lymphocytes, Enzyme-Linked-Immunosorbent-Assay (ELISA), Fluorescent-Linked-Immunosorbent-Assay (FLISA), Western blotting, Dot-and slot-immunoblotting, Immunohistochemistry |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC), rhodamine (TRITC), and Texas Red (TXRD) conjugates are supplied as 1.0 mgin 1.0 mL PBS/NaN3. Store at 2- 8 C - The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C - The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed. |
| Cross Absorption | Rat IgG |
