RayBio® Rat IL-6 ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Best Seller
Rat IL-6 ELISA Kit
Rat IL-6 ELISA Kit
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Rat |
| Sensitivity | 30 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 30 pg/ml - 10000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta-NGF, TIMP-1, TNF-alpha. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P20607 |
| Gene Id | 24498 |
| Gene Symbols | IL6, IL-6 |
| Protein Name / Synonyms | Interleukin-6 (IL-6) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Cardiovascular Disease, Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
| Sample type | Cell Culture Supernatants / Plasma / Serum, Cell and Tissue Lysates |
Related Products
NexaTag® Rat IL-6 qIPCR ELISA Kit
As low as $749.00
Target Background
Interleukin 6 (IL-6) is a pleiotropic cytokine that regulates cell growth and differentiation of various tissues, and plays an important role in the immune response and acute phase reactions. IL-6 acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate an immune response, e.g., during infection and after trauma, especially burns or other tissue damage leading to inflammation. IL-6's role as an anti-inflammatory cytokine is mediated through its inhibitory effects on TNF-alpha and IL-1, and activation of IL-1ra and IL-10.
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Rat IL-6 ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified IL-6 (IL6), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)
citations
Neuroprotective effects of telmisartan in a harmaline-induced model of essential tremor: modulation of the renin-angiotensin system and inflammatory pathways
- Species:
- Rat
- Sample Type:
- Other / Unspecified (cerebella)
- Year:
- 2026
- Journal Name:
- Frontiers in Pharmacology
- PubMed ID:
- 42305442
Evaluation of the protective effect of direct peritoneal resuscitation on lung injury in a controlled hemorrhagic shock model in rats
- Species:
- Rat
- Sample Type:
- Tissue Lysate / Homogenate (left lung tissue)
- Year:
- 2026
- Journal Name:
- Scientific Reports
- PubMed ID:
- 42069909
Dysregulation of protein degradation and alteration of secretome in α-synuclein-exposed astrocytes: implications for dopaminergic neuronal dysfunction
- Species:
- Rat
- Sample Type:
- Cell Culture Supernatant (conditioned media of astrocytes or DA neurons)
- Year:
- 2024
- Journal Name:
- Cell Communication and Signaling
- PubMed ID:
- 39617881
Cytoprotective Effects of Agomelatine on Hepatic Ischemia-Reperfusion Injury in a Rat Model
- Year:
- 2026
- Journal Name:
- International Journal of Molecular Sciences
- PubMed ID:
- 41977427
Behavioral and biochemical evaluation of the neuroprotective role of naringin in streptozotocin‑induced diabetic peripheral neuropathy in rats
- Species:
- Rat
- Sample Type:
- Serum; Tissue Lysate / Homogenate (brain homogenate)
- Year:
- 2026
- Journal Name:
- Exploration of Endocrine and Metabolic Diseases
Exploring the Potential of Transdermal Nanobilosomal Gel for Magnified Anti-Inflammatory Efficacy of Thymol for Managing Rheumatoid Arthritis
- Species:
- Rat
- Sample Type:
- Serum (FCA-induced arthritic wistar rats)
- Year:
- 2026
- Journal Name:
- Gels (Basel, Switzerland)
- PubMed ID:
- 41745028
Modulation of TNFα-driven neuroinflammation by Gardenin A: insights from in vitro, in vivo, and in silico studies
- Species:
- Rat
- Sample Type:
- Tissue Lysate / Homogenate (brain tissue homogenates)
- Year:
- 2025
- Journal Name:
- Frontiers in Pharmacology
- PubMed ID:
- 41293241
Melatonin promotes gut anti-oxidative status in perinatal rat by remodeling the gut microbiome
- Species:
- Rat
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Redox biology
Disease-modifying effects of a glial-targeted inducible nitric oxide synthase inhibitor (1400W) in mixed-sex cohorts of a rat soman (GD) model of epilepsy
- Species:
- Rat
- Sample Type:
- Serum, cerebrospinal fluid (CSF)
- Year:
- 2023
- Journal Name:
- Journal of Neuroinflammation
Equisetum diffusum whole plant methanol extract attenuates rheumatoid arthritis by modulating the expression of key inflammatory biomarkers in rat models
- Species:
- Rat
- Sample Type:
- Serum (FCA-induced arthritis)
- Year:
- 2025
- Journal Name:
- Scientific Reports
- PubMed ID:
- 40121199
