Orb1950133-48Tests | Rat Integrin Alpha 2B (ITGa2B) ELISA Kit Clinisciences

Rat Integrin Alpha 2B (ITGa2B) ELISA Kit

SKU: orb1950133

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Description

The Rat Integrin Alpha 2B (ITGa2B) ELISA Kit uses a sandwich enzyme immunoassay to quantify Integrin Alpha 2B (ITGa2B) in rat serum, plasma, tissue homogenates, cell lysates, breast milk, cell culture supernates and other biological fluids. The kit has a detection range of 125-8000 pg/mL and a sensitivity of 34.9 pg/mL.

Research Area

Signal Transduction

Images & Validation

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Application Notes	standard: 8000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ITGa2B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ITGa2B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ITGa2B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ITGa2B in the samples is then determined by comparing the OD of the samples to the standard curve

Application Notes

standard: 8000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ITGa2B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ITGa2B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ITGa2B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ITGa2B in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	serum, plasma, tissue homogenates, cell lysates, breast milk, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	125-8000 pg/mL
Sensitivity	34.9 pg/mL
Concentration	8000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CD41; CD41B; ITG-A2B; GTA; GP2B; GPIIb; HPA3; Platelet Glycoprotein IIb Of IIb/IIIa Complex; Antigen CD41

Rat Integrin Alpha 2B (ITGa2B) ELISA Kit

Referentie orb1950133-48Tests

Merk : Biorbyt

Rat Integrin Alpha 2B (ITGa2B) ELISA Kit

Description

Research Area

Images & Validation

Key Properties

Storage & Handling

Alternative Names

Protocol Information