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> Recombinant Flap endonuclease 1 (FEN1)

Recombinant Flap endonuclease 1 (FEN1)

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Referentie MBS1225016-0,5mg-Y

Formaat : 0.5mg(Yeast)

Merk : MyBiosource


Flap Endonuclease 1 (FEN1) Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1225016

MBS1225016 is a recombinant protein targeting FEN1. Also known as Recombinant Flap endonuclease 1 (FEN1). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

Product Overview

Online Datasheet in PDF
Product Name
Flap endonuclease 1 (FEN1), Recombinant Protein
Full Product Name
Recombinant Flap endonuclease 1 (FEN1)
Product Gene Name
FEN1 recombinant protein
Product Synonym Gene Name
FEN1; LinJ27.0180; LinJ_27_0260
Product Synonym Names
Flap endonuclease 1; FEN-1; EC=3.1.-.-; Flap structure-specific endonuclease 1
Sequence
MGILGLSKLL YDKSPNAIRE QELKNFFGRR IAIDASMSIY QFIIAMKGFQ DGQGLELTNE KGDVTSHLNG LFARTLRMID EGIKPIYVFD GKPPKLKADE LEMRRQKAAE AEREFEKAKD AGDDEMMEKM SKRTVRVSRD QIDESKKLLR LMGIPVIQAP SEAEAQCAEL VKKGKAWAVG TEDMDALTFG STVMLRHLNI SDAKKRPIAE IHLDEVLQIT GLSMGQFVDL CILLGCDYVP KVPGIGPQKA WEGIQRYGSI ESFLESLDTT KHPVPADFYY KEARAFFQNP EVTPAEEINI QFSEPDEVGL IQFLVKEKLF NPDRVNKGIA RLRAALTRKT QGRLDSFFTV TKVPQQTAAA RAPLAGTKRP RDGKYVHVSG TLRKATSGHK KAVKK
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Molecular Weight
44,201 Da
Sequence Positions
1-395aa; full length protein
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for A4I2L4
Species
Leishmania infantum
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of FEN1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for FEN1. It may not necessarily be applicable to this product.

NCBI GI #
146090657
NCBI GeneID
5070003
NCBI Accession #
XP_001466297.1
UniProt Primary Accession #
A4I2L4
GenBank Nucleotide #
XM_001466260.1
NCBI Official Full Name
putative flap endonuclease-1 (FEN-1)
NCBI Official Symbol
LINJ_27_0260
UniProt Protein Name
Flap endonuclease 1
UniProt Synonym Protein Names
Flap structure-specific endonuclease 1
UniProt Gene Name
FEN1
UniProt Synonym Gene Names
FEN-1
UniProt Entry Name
FEN1_LEIIN
NCBI Protein Information
putative flap endonuclease-1 (FEN-1)

UniProt Comments for FEN1

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with FEN1 recombinant protein

 Products by Pathway  Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 Non-homologous End-joining Pathway antibodies  Non-homologous End-joining Pathway Diagram
 Non-homologous End-joining Pathway antibodies  Non-homologous End-joining Pathway Diagram