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> Recombinant SARS-CoV-2 Nucleocapsid Protein

Recombinant SARS-CoV-2 Nucleocapsid Protein

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Referentie 230-30164-100

Formaat : 100ug

Merk : RayBiotech


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Recombinant SARS-CoV-2 Nucleocapsid ProteinRecombinant SARS-CoV-2 Nucleocapsid Protein

Recombinant SARS-CoV-2 Nucleocapsid Protein

Recombinant SARS-CoV-2 Nucleocapsid Protein

Recombinant SARS-CoV-2 Nucleocapsid Protein with C-terminal His-tag, derived from the transfected human HEK293 cells.
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Product Description

Specifications

Size100 µg
SpeciesSARS-CoV-2
Accession Number
QHD43423
Protein Name / Synonyms
Nucleocapsid Protein, N Protein
Expressed Region
Met1-Ala419
Expression SystemHEK293 cells
TagC-terminal his-tag
Purity
>90 %
Purity Determined BySDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa)
Recombinant protein product has a calculated molecular mass of 47 kDa. Due to the abundant glycosylation, it migrates as approximately 55 kDa major protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. The minor small protein bands (25-30 kDa) likely the cleaved products. See deglycosylation analysis image below.
Format
Liquid
Formulation
Supplied as a 0.2 µm filtered solution in PBS (pH 7.4)
Concentration (lot specific)
Lot specific (see the label on the vial), determined by BCA protein assay
Research AreaInfectious Disease, COVID-19
Shipping TypeBlue ice
Storage-20°C

Introduction

SARS-CoV-2 relies on its nucleocapsid (N) protein to package and protect its RNA genome within the viral particle. Beyond its role in viral replication and assembly, the N protein also interacts with host immune signaling pathways, potentially modulating immune responses and disease severity. Due to its high immunogenicity, it is widely used as a diagnostic target for detecting SARS-CoV-2 infection.

Reference: https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1012886

SDS-PAGE Image
SDS-PAGE
Figure 1. Deglycosylation of purified recombinant proteins. Purified proteins were untreated (Lane 2) or treated with Protein Deglycosylation Kit under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size (47 kDa), thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.
Lane 1: Protein standard ladder (kDa)
Lane 2: Untreated protein under reducing conditions. Shown one ˜55 kDa major band (red arrow) and 25-30 kDa multiple of minor bands (green arrows). This small bands are likely cleavage products. All bands were confirmed by Western blotting (Lane 5). Other minor large bands (>80 kDa) may be the trace amount of copurified proteins from host cells.
Lane 3: Treated protein with deglycosylation enzymes under native conditions. Shown one 47 kDa major band (red arrow) and two minor 22-30 kDa bands (green arrows).
Lane 4: Treated protein with deglycosylation enzymes under reducing conditions. Shown one 47 kDa major band (red arrow) and two minor 22-30 kDa bands (green arrows).
Lane 5: Western blotting analysis of Lane 1. Shown two major reaction bands (red arrow, green arrow).
Activity
Sandwich ELISA: this protein functions as a standard in conjunction with 130-10781 (capture antibody) and 130-10761 (detection antibody).
Standard curve
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.

Custom Labeling Request

We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.

citations

  • Hybrid immunity from bivalent vaccination and prior infection enhances humoral and innate protection against Omicron XBB.1.16 and EG.5.1.1 variants in Japan

    Species:
    Human
    Sample Type:
    Other / Unspecified (monocyte-derived macrophages)
    Year:
    2026
    Journal Name:
    Frontiers in Immunology
    PubMed ID:
    42465771

  • Adenoviral vector oropharyngeal spray immunization elicits mucosal immunity and protects against heterologous SARS-CoV-2 infection

    Year:
    2025
    Journal Name:
    npj Vaccines
    PubMed ID:
    41390777

  • SARS-CoV-2 Seroprevalence of Surinamese Children and Determinants of Seropositivity in the CCREOH/MeKiTamara Cohort

    Species:
    Human
    Sample Type:
    Other / Unspecified (DBS eluate samples)
    Year:
    2026
    Journal Name:
    Children (Basel, Switzerland)
    PubMed ID:
    42073071

  • Rapid quantitation of SARS-CoV-2 antibodies in clinical samples with an electrochemical sensor

    Year:
    2022
    Journal Name:
    Biosensors & Bioelectronics

  • Multiplex quantitative detection of SARS-CoV-2 specific IgG and IgM antibodies based on DNA-assisted nanopore sensing

    Species:
    Human
    Sample Type:
    Other / Unspecified (SARS-CoV-2 IgG, IgM antibody detection assay)
    Year:
    2021
    Journal Name:
    Biosensors & Bioelectronics
    PubMed ID:
    33761415

  • Reaction of SARS-CoV-2 antibodies with other pathogens, vaccines, and food antigens

    Year:
    2022
    Journal Name:
    Frontiers in immunology

  • Ultrasensitive high-resolution profiling of early seroconversion in patients with COVID-19

    Year:
    2020

  • Deconvoluting the T Cell Response to SARS-CoV-2: Specificity Versus Chance and Cognate Cross-Reactivity

    Species:
    Human
    Sample Type:
    Plasma (donor plasma)
    Year:
    2021
    Journal Name:
    Frontiers in Immunology
    PubMed ID:
    34127926

  • SARS-CoV-2 BA.1 variant is neutralized by vaccine booster-elicited serum but evades most convalescent serum and therapeutic antibodies

    Species:
    Human
    Year:
    2022

  • S Protein-Reactive IgG and Memory B Cell Production after Human SARS-CoV-2 Infection Includes Broad Reactivity to the S2 Subunit

    Year:
    2020
    Journal Name:
    Mbio

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