RH IL-4 (AF)
Referentie 100-09AF-5UG
Formaat : 5UG
Merk : FUJIFILM
Recombinant Human IL-4
Interleukin 4 (IL-4) is an immunomodulatory cytokine that functions to induce naïve helper T cells to differentiate into type 2 T helper (Th2) cells. Th2 cells subsequently produce more IL-4 in a positive feedback loop. IL-4 also promotes immunoglobulin IgG to IgE isotype switching on B cells. IL-4 binds the IL-4Rα receptor to activate STAT6 signaling.
Also available as a GMP product: CTGrade GMP rh IL-4
Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.
SDS-PAGE Showing a Prominent Band 14.4 kDa.
Figure 1. rh IL-4 (1μg) was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by InstantBlue staining showing a single prominent band at 14.4 kDa.*
*Predicted MW is 15.1 kDa, but runs on SDS-PAGE at 14.4 kDa.
Cell-Based Proliferation Assay
Figure 2. Recombinant Human IL-4 induced proliferation in TF-1 cells.
Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Technical Specifications
| Alternate Names | B-cell stimulatory factor-1, BSF-1, BCDF, BCGF |
| Accession Number | P05112 |
| Source | Genetically modified E.coli |
| Predicted Molecular Mass | Monomer, 15.1 kDa (130 aa) |
| AA Sequence | MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS |
| Formulation | Lyophilized from a 0.2 µm filtered solution containing 0.1% Trifluoroacetic Acid (TFA) |
| Product Reconstitution | Sterile water at 0.1 mg/mL |
| Country of Origin | USA |
| Specification* | Method of Determination | Acceptance Criteria |
| Purity | Reducing and Non-Reducing SDS PAGE | ≥ 95% |
| Endotoxin | Kinetic LAL | ≤ 0.1 EU/µg |
| Biological Activity (ED50) | TF-1 cell proliferation | ≤ 0.25 ng/mL; ≥ 4.0 x 106 units/mg |
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.