RM IL-1A (AF)
Referentie 200-87AF-5UG
Formaat : 5UG
Merk : FUJIFILM
Recombinant Mouse IL-1α / IL-1F1
Interleukin 1 alpha (IL-1α) is expressed by epithelial cells, activated macrophages, neutrophils, and endothelial cells to regulate immune responses. IL-1α signals through the IL-1 receptor, type 1 (IL-1R1) to activate the myeloid differentiation primary response 88 (MYD88) signaling pathway, which contains the cytoplasmic Toll/IL-1 receptor (TIR) domain adapter. IL-1α and the independently regulated IL-1β protein have overlapping proinflammatory activities to induce adhesion molecule expression on epithelial cells, control fever induction, initiate rheumatoid arthritis, and promote septic shock.
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SDS-PAGE Showing a Single Prominent Band at 18.1 kDa
Figure 1. rm IL-1 alpha (1μg) was resolved with SDS-PAGE under non-reducing and reducing (R) conditions and visualized by Instant Blue staining showing a single prominent band at 18.1 kDa.
Cell based proliferation assay
Figure 2. Recombinant Mouse IL-1α / IL-1F1 induced proliferation in D10.G4.1 cells
Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Technical Specifications
| Alternate Names | None |
| Accession Number | P01582 |
| Source | Genetically modified E.coli |
| Predicted Molecular Mass | Monomer, 18.1 kDa (157 aa) |
| AA Sequence | MSAPYTYQSD LRYKLMKLVR QKFVMNDSLN QTIYQDVDKH YLSTTWLNDL QQEVKFDMYA YSSGGDDSKY PVTLKISDSQ LFVSAQGEDQ PVLLKELPET PKLITGSETD LIFFWKSINS KNYFTSAAYP ELFIATKEQS RVHLARGLPS MTDFQIS |
| Formulation | Lyophilized from a 0.2 µm filtered solution containing 10 mM sodium phosphate, pH 7.5 |
| Product Reconstitution | Sterile water at 0.1 mg/mL |
| Country of Origin | USA |
| Specification* | Method of Determination | Acceptance Criteria |
| Purity | Reducing and Non-Reducing SDS PAGE | ≥ 95% |
| Endotoxin | Kinetic LAL | ≤ 0.1 EU/µg |
| Biological Activity (ED50) | D10.G4.1 proliferation | ≤ 0.01 ng/mL |
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.