Terminal Deoxynucleotidyl Transferase (TdT)
Referentie MBS9719251-5x0,1mg
Formaat : 5x0.1mg
Merk : MyBiosource
Fig. SDS-PAGE analysis of TdT.
Formulation
Fluorescence images using TdT and Fluorescence-labeled dUTP in Hela cells with the treatment of 10 U/mL Dnase I for 10min. The cells were counterstained with DAPI.
Terminal Deoxynucleotidyl Transferase (TdT) Recombinant Protein
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9719251Description: Terminal Deoxynucleotidyl Transferase (TdT), expressed in NullBackground: Terminal deoxynucleotidyl transferase (TdT), also known as terminal transferase, is a specialized DNA polymerase expressed in immature, pre-B, pre-T lymphoid cells, and… More details
Product Overview
Online Datasheet in PDFProduct Name
Terminal Deoxynucleotidyl Transferase (TdT), Recombinant Protein
Full Product Name
Terminal Deoxynucleotidyl Transferase (TdT)
Product Gene Name
TdT recombinant protein
Product Synonym Names
TdT; DNA nucleotidylexotransferase; Terminal addition enzyme; Terminal deoxynucleotidyltransferase; Terminal transferase
Host
E. coli
Form/Format
The recombinant bovine TdT reconstitution solution, the compound solution is a liquid in sterile aqueous solution, 50 mM K2HPO4, 100 mM NaCl, 1 mM DTT, 0.1% Tween20, 1% BSA, 50% Glycerol, pH6.5.
Purity/Purification
> 90% as determined by SDS-PAGE
Preparation and Storage
Store at -20 degree C.
Molecular Weight
58,296 Da
Tag
TdT
Protein Construction
The recombinant bovine TdT consists of 509 amino acids and migrates with an apparent molecular mass of 58.3kDa as estimated in SDS-PAGE under reducing conditions.
Unit Definition
One unit of the enzyme catalyzes the incorporation of 1 nmol of deoxythymidylate into a polynucleotide fraction in 60 min at 37°C.
Usage Notes
The Equilibration Buffer (5×) stock solution is stored at low temperature, resulting in a small amount of component precipitation. Please invert and mix before use. And Equilibration Buffer (5×) contains cacodylate and cobalt chloride, highly toxic chemicals. After contact with skin, wash immediately with plenty of water. In case of accident or if you feel unwell, seek medical advice immediately.
Product Categories/Family for TdT recombinant protein
Cytokines; Cell Status Detection; Cellular states; Cytology
Other Notes
Small volumes of TdT recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for TdT purchase
MBS9719251 is a recombinant protein targeting TdT. Also known as Terminal Deoxynucleotidyl Transferase (TdT). Host: E. coli.
Additional Details
NCBI/Uniprot data below describe general gene information for TdT. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
DNA nucleotidylexotransferase
NCBI Official Synonym Full Names
DNA nucleotidylexotransferase
NCBI Official Symbol
DNTT
UniProt Protein Name
DNA nucleotidylexotransferase
UniProt Synonym Protein Names
Terminal addition enzyme; Terminal deoxynucleotidyltransferase; TDT; Terminal transferase
Protein Family
Tonoplast dicarboxylate transporter
UniProt Gene Name
DNTT
UniProt Synonym Gene Names
TDT; TDT; Terminal transferase
NCBI Protein Information
DNA nucleotidylexotransferase
UniProt Comments for TdT
Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
Show more Research Articles on TdT
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Pathways associated with TdT recombinant protein
| Products by Pathway | Pathway Diagram |
|---|---|
| Hematopoietic Cell Lineage Pathway antibodies | Hematopoietic Cell Lineage Pathway Diagram |
| Hematopoietic Cell Lineage Pathway antibodies | Hematopoietic Cell Lineage Pathway Diagram |
| Non-homologous End-joining Pathway antibodies | Non-homologous End-joining Pathway Diagram |
| Non-homologous End-joining Pathway antibodies | Non-homologous End-joining Pathway Diagram |
Diseases associated with TdT recombinant protein
| Disease Name | Pubmed Publications |
|---|---|
| Lymphoma Antibodies | >2 publications with TdT and Lymphoma |