4430A | TMB ONE-Prestained Clinisciences

Three brown laboratory bottles in different sizes with Kementec Solutions TMB ONE-Prestained and ELISA Plate Blocking

Graph illustrating sensitivity and speed og the different TMB formulations

Graph illustrating the comparison of TMB and OPD tablets

Graph illustrating the stability of TMB ONE over time

TMB (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP, and the addition of a stop solution gives a clear yellow color that absorbs at 450 nm. This substrate gives higher signal and has a higher sensitivity in ELISA than OPD (ortho-phenylenediamine dichloride).

TMB ONE characteristics are:

“The Gold Standard” used across the industry
Incredibly stable and robust
Aqueous based – no organic solvents (REACH Annex XVII compliant)
Low lot-to-lot variation
Suitable for use in electrochemical assays

For traceable pipetting, we recommend using pink colored TMB ONE Prestained (cat. no. 4430). The pink indicator disappears after addition of the stop solution.

The product is available in bulk quantities and as customized solutions.