ASM10194-A680-100 | TrpM7 Antibody Clinisciences

Immunohistochemistry analysis using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74-25 (ASM10194). Tissue: Brain Slice. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody (ASM10194) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Localization: Nuclear staining of both neurons and glia.
Immunohistochemistry analysis using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74-25 (ASM10194). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody (ASM10194) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
Western Blot analysis of Human Cell lysates showing detection of TrpM7 protein using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74-25 (ASM10194). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody (ASM10194) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, IHC, ICC/IF, AM
Primary Accession	Q923J1
Other Accession	NP_001157797.1
Host	Mouse
Isotype	IgG1
Reactivity	Human, Mouse, Rat
Clonality	Monoclonal
Description	Mouse Anti-Mouse TrpM7 Monoclonal IgG1
Target/Specificity	Detects ~220kDa. No cross-reactivity against TrpM6.
Other Names	CHAK antibody, CHAK1 antibody, Channel kinase 1 antibody, Channel-kinase 1 antibody, Long transient receptor potential channel 7 antibody, LTrpC-7 antibody, LTRPC7 antibody, Transient receptor potential cation channel subfamily M member 7 antibody, TRP PLIK antibody, TRPM7 antibody, TRPM7_HUMAN antibody
Clone Names	S74-25
Immunogen	Fusion protein amino acids 1817-1863 (C- terminus) of mouse TrpM7
Purification	Protein G Purified
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	1 µg/ml of SMC-316 was sufficient for detection of TrpM7 in 10 µg of COS cell lysate transiently transfected with TprM7 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization	Membrane

TrpM7 Antibody