Ulex europaeus (UEA I, Gorze, Furze) (FITC)

Ulex europaeus agglutinin I is a glycoprotein with a molecular weight of 63,000, although multimeric aggregates have been reported. UEA I has two subunits, one of about 31,000D and another of 32,000D. UEA I binds to many glycoproteins and glycolipids containing a -linked fucose residues, such as ABO blood group glycoconjugates. This lectin preferentially binds blood group O cells and has been used to determine secretor status. It has been established as an excellent marker for human endothelial cells. Fluorescein labeled Ulex Europaeus Agglutinin I is produced by using the highest quality fluorescein isothiocyanate, our affinity-purified lectin, and special conjugation procedures. Fluorescein labeled Ulex Europaeus Agglutinin I has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This lectin is supplied essentially free of unconjugated fluorochromes and inactive lectin. Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions perserved with sodium azide.

Applications:
Suitable for use in Immunofluorescence. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Inhibiting/Eluting Sugar:
50mM-100mM L-fucose

Excitation:
495nm

Emission:
515nm

F/P (molar):
2.4

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 6 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.