A6005-5mg | V5 Epitope Tag Peptide Clinisciences

Background

The recognized V5 epitope represents ⁹⁵GKPIPNPLLGLDST¹⁰⁸ of RNA polymerase α subunit of simian parainﬂuenza virus type 5. This short peptide sequence was chosen because high-afﬁnity antibodies can be reliably produced in many different species¹.

A 14-amino-acid V5 epitope derived from simian parainﬂuenza virus type 5 (Southernet al., 1991) was inserted into the C terminus of the capsid protein (before the stop codon ofORF2) to construct a recombinant marker virus. We demonstrated that the V5 epitope was displayed on the surface ofthe capsid protein. Furthermore, the recombinant marker virus behaved similarly to the parental virus in vitro and in mice, and could be differentiated from the parental virus via polymerase chain reaction (PCR) and serological methods^{2, 3}.

A V5/His-epitope tag was added to the amino terminus of each coding sequence to distinguish the plasmid-encoded protein from endogenous LRRK2. Plasmids were transiently transfected into COS7 cells, and cell lysates were analyzed by SDS-PAGE and Western blotting with antibodies speciﬁc for the V5 epitope tag or LRRK2⁴.

References:
1. Zhou,W.H.,Dong, L.L., Ginsburg,D.,Bouhassira, E.E., Tsai,H.M.,2005. Enzymatically active ADAMTS 13 variants are not inhibited by anti-ADAMTS 13 autoantibodies: a novel therapeutic strategy? J. Biol. Chem. 280, 39934–39941.
2. Southern,J.A.,Young,D.F.,Heaney, F.,Baumgartner,W.K.,Randall,R.E.,1991.Identiﬁcation of an epitope on the P and V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics. J. Gen. Virol. 72,1551–1557
3. L. Huang, Y. Lu et al. Construction and biological characterisation of recombinant porcine circovirus type 2 expressing the V5 epitope tag. Virus Research. 161 (2011) 115–123
4. D. MacLeod,J. Dowman, The Familial Parkinsonism Gene LRRK2Regulates Neurite Process Morphology. Neuron 52, 587–593, 2006

Product Citation

1. Takushi Miyoshi, Qianli Zhang, et al. "Semi-automated single-molecule microscopy screening of fast-dissociating specific antibodies directly from hybridoma cultures." Cell Rep. 2021 Feb 2;34(5):108708. PMID: 33535030
2. Yu Z, Surface LE, et al. "Identification of a transporter complex responsible for the cytosolic entry of nitrogen-containing-bisphosphonates." Elife. 2018 May 10;7. pii: e36620. PMID: 29745899

Chemical Properties

Physical Appearance	A solid
Storage	Desiccate at -20°C
M.Wt	1421.64
Formula	C₆₄H₁₀₈N₁₆O₂₀
Synonyms	H-Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-OH
Solubility	≥71.08 mg/mL in DMSO; ≥107.2 mg/mL in EtOH; ≥55.4 mg/mL in H2O
Chemical Name	V5 Epitope Tag
SDF	Download SDF
Canonical SMILES	CCC(C)C(C(=O)N1CCCC1C(=O)NC(CC(=O)N)C(=O)N2CCCC2C(=O)NC(CC(C)C)C(=O)NC(CC(C)C)C(=O)NCC(=O)NC(CC(C)C)C(=O)NC(CC(=O)O)C(=O)NC(CO)C(=O)NC(C(C)O)C(=O)O)NC(=O)C3CCCN3C(=O)C(CCCCN)NC(=O)CN
Shipping Condition	Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips	We do not recommend long-term storage for the solution, please use it up soon.

Biological Activity

Description	V5 Epitope Tag Peptide is a tag peptide derived from a small epitope present on the P and V proteins of the paramyxovirus of simian virus 5.
Targets	Anti-V5 tag antibody
IC50

Quality Control

Purity = 99.67%

Chemical structure

Related Biological Data

V5 Epitope Tag Peptide

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