ASM10540-A633-100 | VMP1 Antibody Clinisciences

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-VMP1 Polyclonal Antibody (ASM10540). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-VMP1 Polyclonal Antibody (ASM10540) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane, Vacuole Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) VMP1 Antibody. (D) Composite.
Western blot analysis of Rat Pancreas cell lysates showing detection of 46.2kDa VMP1 protein using Rabbit Anti-VMP1 Polyclonal Antibody (ASM10540). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Pancreas cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-VMP1 Polyclonal Antibody (ASM10540) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat-Anti-Rabbit IgG: HRP at 1:200 for 60 min at RT. Color Development: TMB. Predicted/Observed Size: 46.2kDa. Other Band(s): Multiple bands for A549; Rat Brain shows a band at 60kDa, 75kDa, and 150kDa.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, ICC/IF
Primary Accession	Q96GC9
Other Accession	NP_112200.2
Host	Rabbit
Reactivity	Human
Clonality	Polyclonal
Description	Rabbit Anti-Human VMP1 Polyclonal
Target/Specificity	Detects ~46 kDa.
Other Names	TDC1 Antibody, Transmembrane protein 49 Antibody, TMEM49 Antibody, VMP1 Antibody, HSPC292 Antibody, Vacuole membrane protein 1 Antibody, ORF Names: Antibody
Immunogen	Synthetic peptide from the C-terminal of Human VMP1 (aa. 391-402)
Purification	Peptide Affinity Purified
Storage	-20ºC
Storage Buffer	PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	A 1:1000 dilution of SPC-680 was sufficient for detection of VMP1 in 15 µg of Human 293T Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization	Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane \| Multi-Pass Membrane Protein \| Cell Membrane \| Multi-Pass Membrane Protein \| Vacuole Membrane \| Multi-Pass Membrane Protein \| Endoplasmic Reticulum

VMP1 Antibody