West Nile Virus IgM ELISA Kit

Background: Besides the Dengue virus, the Yellow Fever virus and the TBE virus, the West Nile virus belongs to the human pathogenic species among the flaviviruses. Birds such as ravens and sparrows represent the natural reservoires for the arbovirus (arthropode borne virus). Mosquitos of the genus Culex, Aedes and Ochlerotatus are the main vectors of the West Nile virus to humans. ELISA test systems are especially suited for the differential analysis of immunoglobulin classes directed against the virus.

Principle of the Assay: The ELISA (Enzyme Linked Immunosorbent Assay) is an immunoassay, which is particularly suited to the determination of antibodies in various kinds of samples. The reaction is based on the specific interaction of antibodies with their corresponding antigen. The test strips of the microtiter plate are coated with specific antigens of the pathogen of interest. If antibodies in the sample are present, they bind to the fixed antigen. A secondary antibody, which has been conjugated with the enzyme alkaline phosphatase, detects and binds to the immune complex. The colorless substrate pnitrophenylphosphate is then converted into the coloured product p-nitrophenol. The signal intensity of this reaction product is proportional to the concentration of the analyte in the sample and is measured photometrically.

Intended Uses: The West Nile Virus IgG and IgM tests are qualitative immunoassays for the demonstration of human antibodies in serum or plasma directed against West Nile virus.

Small volumes of IgM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS495364 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the West Nile Virus IgM, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IgM. The ELISA analytical biochemical technique of the MBS495364 kit is based on IgM antibody-IgM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IgM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IgM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).