ASM10525-P594-100 | WIPI2 Antibody Clinisciences

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-WIPI2 Polyclonal Antibody (ASM10525). Tissue: HeLa Cells (Human Cervical Cancer). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-WIPI2 Polyclonal Antibody (ASM10525) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) WIPI2 Antibody (D) Composite.
Western blot analysis of Human HeLa and 293Trap cell lysates showing detection of ~49.4 kDa WIPI2 protein using Rabbit Anti-WIPI2 Polyclonal Antibody (ASM10525). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Lane 3: 293Trap cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-WIPI2 Polyclonal Antibody (ASM10525) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~49.4 kDa.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, ICC/IF
Primary Accession	Q9Y4P8
Other Accession	NP_001028690.1
Host	Rabbit
Reactivity	Human
Clonality	Polyclonal
Description	Rabbit Anti-Human WIPI2 Polyclonal
Target/Specificity	Detects ~55 kDa.
Other Names	WIPI2 Antibody, WD repeat domain phosphoinositide-interacting protein 2 Antibody, WIPI-2 Antibody, WIPI49-like protein 2 Antibody, CGI-50 Antibody, ATG21 Antibody, WIPI 2 Antibody, ATG18B Antibody
Immunogen	Synthetic peptide from the C-terminal of Human WIPI2
Purification	Peptide Affinity Purified
Storage	-20ºC
Storage Buffer	PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	A 1:1000 dilution of SPC-649 was sufficient for detection of WIPI2 in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization	Preautophagosomal Structure Membrane \| Peripheral Membrane Protein \| Cytoplasmic Side

WIPI2 Antibody