Reagentia en instrumenten voor immunologie, celbiologie en moleculaire biologie.

Wnt-C59 [1243243-89-1]

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Referentie NB-64-11677-2mg

Formaat : 2mg

Merk : Neo Biotech

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Wnt-C59

(Synonyms: C59) Copy Product Info

Synonyms: C59

Catalog No. T2242 Copy Product Info

Purity: 99.97%

Wnt-C59 (C59) is a highly effective and specific Wnt signaling antagonis with PORCN enzymatic activity.

Cas No. 1243243-89-1

With extensive experience in compound synthesis, we can provide rapid custom synthesis services for this product according to your research needs.

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.97%

Color:White

Product Introduction

Bioactivity

Description	Wnt-C59 (C59) is a highly effective and specific Wnt signaling antagonis with PORCN enzymatic activity.
Targets & IC50	Porcn:74 pM
In vitro	In female nude mice with independently transplanted in-situ MMTV-WNT1 tumors, Wnt-C59 (10 mg/kg) effectively reduces the expression of β-catenin-targeted genes and decreases Wnt pathway activity, suppressing tumor cell growth. Additionally, in 14CREER/Rosa-SmoM2 mice, topical administration of Wnt-C59 (5 mg/kg) inhibits cell proliferation.
In vivo	In PORCN-deficient HT1080 cells transfected with PORCN, Wnt-C59 (100 nM) inhibits PORCN activity. Similarly, in HeLa cells transfected with WNT3A-V5, Wnt-C59 (10 -100 nM) suppresses the activity of the PORCN acyltransferase.
Synonyms	C59
Kinase Assay	Aurora A radioactive Flashplate enzyme assay: Aurora A radioactive Flashplate enzyme assay is conducted to determine the nature and degree of MLN8237-mediated inhibition in vitro. Recombinant Aurora A is expressed in Sf9 cells and purified with GST affinity chromatography. The peptide substrate for Aurora A is conjugated with biotin (Biotin-GLRRASLG). Aurora A kinase (5 nM) is assayed in 50 mM Hepes (pH 7.5), 10 mM MgCl2, 5 mM DTT, 0.05% Tween 20, 2 μM peptide substrate, 3.3 μCi/mL [γ-33P]ATP at 2 μM, and increasing concentrations of MLN8237 by using Image FlashPlates.
Cell Research	Wnt-C59 (C59) is dissolved in DMSO (10 mM) and stored, and then diluted with appropriate medium before use[1]. Approximately, 1×104 cells are seeded in 24-well plates, and Wnt-C59 (5 μM, 10 μM, and 20 μM) is added the next day. Each group is tested in triplicate and control groups with addition of DMSO are also established. Cell confluence is determined by microscopy at 24, 48, 72, and 96 hours after seeding of cells. The IC50 of Wnt-C59 is determined by MTT assay, using 96-well dishes. Next day, various concentrations of Wnt-C59 are added, and cellular viabilities are measured by a spectrophotometer at both 24 and 48 hours. For sphere formation, approximately one hundred cells are seeded onto the Low Cell Bind Surface 24-well Nunc dish. Each group is done in triplicate and each well had 2 mL medium. Media are changed twice a week, and only half of the media is changed each time. Approximately, 1×103 cells are seeded for each well in the sphere inhibition assay. At 1 to 5 days after plating, all tested cells formed small spheres. Five days later, Wnt-C59 (1 μM, 5 μM, and 20 μM) is added into experimental groups. Abilities for cell growth and sphere images are compared and recorded at the end of the first, second, and third weeks after addition of Wnt-C59, or DMSO in control groups. The sphere growths are observed and recorded daily under microscopy, and the area of spheres is analyzed using Metamorph and recorded as average area (μm2)[1].

Chemical Properties

Molecular Weight	379.45
Formula	C₂₅H₂₁N₃O
Cas No.	1243243-89-1
Smiles	Cc1cc(ccn1)-c1ccc(CC(=O)Nc2ccc(cc2)-c2cccnc2)cc1
Relative Density.	1.198 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Store at low temperature,Keep away from direct sunlight Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Ethanol: 7.6 mg/mL (20.03 mM), Sonication is recommended.

DMSO: 38.32 mg/mL (100.99 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (5.27 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	2.6354 mL	13.1770 mL	26.3539 mL	131.7697 mL
5 mM	0.5271 mL	2.6354 mL	5.2708 mL	26.3539 mL
10 mM	0.2635 mL	1.3177 mL	2.6354 mL	13.1770 mL
20 mM	0.1318 mL	0.6588 mL	1.3177 mL	6.5885 mL

DMSO

	1mg	5mg	10mg	50mg
50 mM	0.0527 mL	0.2635 mL	0.5271 mL	2.6354 mL
100 mM	0.0264 mL	0.1318 mL	0.2635 mL	1.3177 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

Calculator

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Keywords

βcateninWnt-C-59Wnt-C59WntC59Wnt/β-cateninWnt/betacateninWnt/b-cateninWnt C59WntPorcnC-59C 59beta-cateninbetacateninbcatenin

Related Tags: Wnt-C59 chemical structure | Wnt-C59 in vivo | Wnt-C59 in vitro | Wnt-C59 formula | Wnt-C59 molecular weight