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> Xylobiose [6860-47-5]

Xylobiose [6860-47-5]

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Referentie HY-N2468-5mg

Formaat : 5mg

Merk : MedChemExpress


Description

Xylobiose (1,4-β-D-Xylobiose; 1,4-D-Xylobiose) is an orally active Claudin 2/CLDN2 inhibitor and HSP27 inducer. Xylobiose works by regulating intestinal barrier function and glucose and lipid metabolism-related signaling pathways. Xylobiose inhibits CLDN2 expression to reduce intestinal permeability, induces HSP27 to enhance cell protection, and regulates the miR-122a/miR-33a axis to inhibit liver lipid synthesis and improve insulin resistance. Xylobiose can strengthen intestinal barrier integrity, reduce blood sugar and blood lipid levels, and reduce oxidative stress and inflammatory response. Xylobiose can be used in the study of type 2 diabetes and metabolic syndrome[1][2].

IC50 & Target

Claudin-2

 

HSP27

 

In Vitro

Xylobiose (5-20 mg/mL; 3-24 h) down-regulates the gene and protein expression of Claudin 2 in human intestinal Caco-2 cells, up-regulates the gene and protein expression of heat shock proteins HSP27 and HSP70, and enhances the transepithelial electrical resistance (TER) of the cell monolayer, which may strengthen the intestinal barrier function by regulating signaling pathways such as PI3K[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Xylobiose Related Antibodies

RT-PCR[1]

Cell Line: Human intestinal Caco-2 cells
Concentration: 5, 10, 20 mg/mL
Incubation Time: 3, 6, 12, 24 h
Result: Downregulated CLDN2 expression, which is involved in paracellular permeability, and upregulated HSP27 and HSP70 expression, which are cytoprotective proteins. These changes indicated an improvement in the cell's functional state rather than a direct impact on cell viability.
In Vivo

Xylobiose (5% replacement of dietary sucrose; dietary intake; daily; 6 weeks) improves glucose tolerance in the db/db mouse diabetic model, reduces fasting blood glucose, HbA1c, insulin levels and HOMA-IR, reduces plasma total triglycerides, regulate liver miR-122a and miR-33a expression, inhibits liver lipid production-related gene expression, and reduces liver oxidative stress and inflammatory response[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:
Dosage:
Administration:
Result: Animal Model: C57BL/KsJ-db/db male mice (17-24 g, 5-week-old), type 2 diabetes model induced by genetic factors[2] - Dosage (solvent): 5% xylobiose replacing sucrose in the diet (dissolved in the diet) - Administration: Dietary intake, daily, for 6 weeks - Result: Did not affect the body weight change compared with the diabetic control group, while significantly reduced food and water intake.
Masse moléculaire

282.24

Formule

C10H18O9
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C[C@H](O)[C@@H](O)[C@@H](CO)O[C@H]1[C@@H]([C@H]([C@H](O)CO1)O)O
Structure Classification
Initial Source
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvant et solubilité
In Vitro: 

H2O : ≥ 125 mg/mL (442.89 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.5431 mL 17.7154 mL 35.4308 mL
5 mM 0.7086 mL 3.5431 mL 7.0862 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (354.31 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Pureté et documentation
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