EnzyChrom™ Aconitase Assay Kit

Referentie EACO-100

Formaat : 100tests

Merk : BioAssay Systems

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Telefoonnummer : +1 850 650 7790

EnzyChrom™ Aconitase Assay Kit



Application

  • For quantitative determination of aconitase enzyme activity and high-throughput screening assays for aconitase modulator.

Key Features

  • Fast and sensitive. Linear detection range (20 µL sample): 0.5 to 100 U/L for 20 min reaction.
  • Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

  • OD565nm

Samples

  • Biological samples (e.g. cell lysate, tissue homogenate, serum, etc.)

Species

  • All

Procedure

  • 30 min

Size

  • 100 tests

Detection Limit

  • 0.5 U/L

Shelf Life

  • 6 months

More Details

ACONITASE (ACONITATE HYDRATASE) is an enzyme in the citric acid (TCA) cycle that catalyzes the conversion of citrate to isocitrate. The activity of aconitase depends largely upon the iron-sulfur [Fe4S4] 2+ cluster. Related diseases include aconitase deficiency (e.g. myopathy and exercise intolerance), Friedreich’s ataxia, and diabetes. BioAssay Systems’ aconitase assay measures the isocitrate generated as a product of the aconitase reaction. The isocitrate is then oxidized producing NADPH and the oxidation product. The NADPH converts the dye to an intense violet color with an absorption maximum of 565 nm. The increase in absorbance at 565 nm is directly proportional to aconitase activity.

No frequently asked questions for this new product. Please check back later.

For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.

Stein, C. S., Jadiya, P., Zhang, X., McLendon, J. M., Abouassaly, G. M., Witmer, N. H. & Boudreau, R. L. (2018). Mitoregulin: A lncRNA-encoded microprotein that supports mitochondrial supercomplexes and respiratory efficiency. Cell reports, 23(13), 3710-3720. Assay: Aconitase in human tissue.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Aconitase Assay Kit
Catalog No: EACO-100 Categories Diabetes & Obesity, Enzyme Activity, Metabolism, Oxidative Stress Tags Aconitase assay kits, Aconitase determination kits, Aconitase measurement kits, Aconitase test kits, Diabetes & Obesity, EACO-100, EnzyChrom Aconitase Assay Kits, EnzyChromTM Aconitase Assay Kit, Enzyme Activity, metabolism, Oxidative Stress

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Referentie
Beschrijving
Cond.
Prijs excl. BTW
CHE0019-096
 96wells