PE/Atto 594-conjugated anti-guinea pig primary antibodies are tandem fluorescent reagents designed for the direct detection of guinea pig immunoglobulins in multiplex immunological assays. These conjugates utilize Förster Resonance Energy Transfer (FRET), in which the phycoerythrin (PE) donor transfers excitation energy to the Atto 594 acceptor, producing bright fluorescence with the excitation characteristics of PE and the emission profile of Atto 594.
Properties of the Conjugate
PE/Atto 594-conjugated anti-guinea pig antibodies are typically affinity-purified polyclonal antibodies that provide high specificity for guinea pig IgG. The PE fluorophore offers exceptional brightness through its high molar extinction coefficient, while Atto 594 contributes excellent photostability and intense fluorescence, making the conjugate well suited for sensitive detection and high-resolution fluorescence imaging.
Applications
- Multiparametric analysis: Ideal for multicolor flow cytometry and fluorescence-based assays requiring simultaneous detection of multiple targets.
- Broad application range: Validated for flow cytometry, immunofluorescence, immunohistochemistry, immunocytochemistry, and Western blotting.