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> Alkaline Phosphatase Conjugate Stabilizer, 1X

Alkaline Phosphatase Conjugate Stabilizer, 1X

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Referentie orb623176-1l

Formaat : 1l

Merk : Biorbyt

Alkaline Phosphatase Conjugate Stabilizer, 1X

Alkaline Phosphatase Conjugate Stabilizer, 1X

SKU: orb623176

Description

Alkaline Phosphatase Conjugate Stabilizer is a proprietary formulation to stabilize and dilute alkaline phosphatase conjugates for use in ELISA, Western blot, and other immunoassay applications. This formulation minimizes non-specific interactions and reduces background.

Research Area

Signal Transduction

Images & Validation

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Alkaline Phosphatase Conjugate Stabilizer, 1X
Application Notes
1. Allow product to equilibrate to room temperature (25°C) prior to use. 2. Add 100 µL to each well. When reacting with peroxidase, a soluble blue reaction product develops that can be read at 370 nm or in a range of 620-650 nm. The reaction product is stable for 1 hour. 3. The reaction can be stopped using an appropriate stop solution, such as Stop Solution for TMB Substrates (STOPT), for endpoint assays. STOPT changes the chromogen from blue to yellow and the absorbance to 450 nm. For best results, sample absorbance should be monitored and read before values exceed 2.0 OD units. STOPT can increase the absorbance up to 3-fold, so the reaction should be stopped when absorbance values reach approximately 0.7 OD units.

Key Properties

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Storage & Handling

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Storage2-8°C
Concentration1 X
Expiration Date12 months from date of receipt.
DisclaimerFor research use only
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Protocol Information