Alkaline Phosphatase (Germ type) /ALPG Protein, Rat, Recombinant (His)

Referentie NB-64-54374-100ug

Formaat : 100ug

Merk : Neo Biotech


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Alkaline Phosphatase (Germ type) /ALPG Protein, Rat, Recombinant (His)

(Synonyms: PLAP-like, GCAP, ALPPL2, ALPPL, ALPG, ALP-1, Alkaline phosphatase, placental-like, Alkaline Phosphatase) Copy Product Info

Synonyms: PLAP-like, GCAP, ALPPL2, ALPPL, ALPG, ALP-1, Alkaline phosphatase, placental-like, Alkaline Phosphatase

Catalog No. TMPK-00639 Copy Product Info
Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. Alkaline Phosphatase (Germ type) /ALPG Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 54.10 kDa and the accession number is F1M8U7.
For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:> 95%
Appearance:Solution
Color:Transparent
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Product Introduction

Bioactivity
Bioactivity
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. Alkaline Phosphatase (Germ type) /ALPG Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 54.10 kDa and the accession number is F1M8U7.
Species
Rat
Expression System
HEK293 Cells
TagC-His
Accession NumberF1M8U7
ConstructionVal22-Pro510
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Alkaline Phosphatase (Germ type) /ALPG Protein, Rat, Recombinant (His),Alkaline Phosphatase (Germ type) /ALPG Protein, Rat, Recombinant (His)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsPLAP-like, GCAP, ALPPL2, ALPPL, ALPG, ALP-1, Alkaline phosphatase, placental-like, Alkaline Phosphatase
Research Background
Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
Chemical Properties
Molecular Weight54.10 kDa (predicted). Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

ALPPL-2ALPPL 2ALP1ALP 1
Related Tags: Alkaline Phosphatase (Germ type) /ALPG Protein, Rat, Recombinant (His) chemical structure | Alkaline Phosphatase (Germ type) /ALPG Protein, Rat, Recombinant (His) molecular weight