ELISA of mouse immunoglobulins shows RM220 reacts to mouse IgA. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG2c, IgG3, IgM, IgE, or human IgA. The plate was coated with 50 ng/well of different immunoglobulins. 200ng/mL, 50 ng/mL, or 10 ng/mL of RM220 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. A titer ELISA of mouse IgA. The plate was coated with different amount of mouse IgA. A serial dilution of RM220 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. Western blot of nonreduced (-) and reduced (+) mouse IgA, using 0.5 µg/mL of RevMAb Clone RM220. This antibody reacts to nonreduced IgA.
Product Details Product Name Anti-Mouse IgA Rabbit Monoclonal Antibody, Clone RM220 Product Description Rabbit monoclonal to Mouse IgA Catalog No. 31-1099-00 Clone Name RM220 Product Data Sheet Anti-Mouse IgA Rabbit Monoclonal Antibody Data Sheet RM220
Specificity RM220 specifically reacts to mouse IgA. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG2c, IgG3, IgM, IgE, or human IgA. Immunogen Mouse IgA Application ELISA
Western Blot (WB) (nonreduced)
Species Reactivity Mouse Conjugate None Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide Volume/Item Size 100 µg Concentration 1.0 mg/mL Purity Protein A affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Rabbit IgG Usage ELISA: 0.005 µg/mL – 0.2 µg/mL;
Western Blot (WB): 0.5 µg/mL - 2 µg/mL. Keywords . Product Images
Anti-Mouse IgA rabbit monoclonal antibody [RM220]
Referentie 31-1099-00
Formaat : 100ug
Merk : RevMab Biosciences
Rabbit monoclonal to Mouse IgA
100 µg
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