Anti-Notch 1 Antibody (3W929)
Referentie NB-64-120518-50uL
Formaat : 50uL
Merk : Neo Biotech
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Anti-Notch 1 Antibody (3W929)
(Synonyms: TAN1, notch 1, hN1, AOVD1, AOS5) Copy Product InfoSynonyms: TAN1, notch 1, hN1, AOVD1, AOS5
Catalog No. TMAH-00820 Copy Product Info
Anti-Notch 1 Antibody (3W929) is an antibody targeting Notch 1. Anti-Notch 1 Antibody (3W929) can be used in ELISA,IHC,WB.
For research use only—not for human use. No sales to individuals. Use as intended only.
Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Stability & Storage
| Description | Anti-Notch 1 Antibody (3W929) is an antibody targeting Notch 1. Anti-Notch 1 Antibody (3W929) can be used in ELISA,IHC,WB. |
| Synonyms | TAN1, notch 1, hN1, AOVD1, AOS5 |
| Ig Type | Rabbit IgG |
| Clone | 3W929 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: HepG2 whole cell lysate, THP-1 whole cell lysate, HL-60 whole cell lysate -All lanes: Notch1 antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 273 kDa -Observed band size: 120 kDa 2. IHC image of TMAH-00820 diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| Application | ELISA IHC WB |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cell membrane; Single-pass type I membrane protein.; [Notch 1 intracellular domain]: Nucleus. |
| Tissue Specificity | In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4(+) and CD8(+) cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO). |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human Notch1 |
| Antigen Species | Human |
| Gene Name | NOTCH1 |
| Gene ID | 4851 |
| Uniprot ID | P46531 |
| Biology Area | Epigenetics and Nuclear Signaling, Neuroscience, Cardiovascular, Developmental biology, Stem cells |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Keywords
TAN-1TAN 1AOVD-1AOVD 1AOS-5AOS 5
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