Reagentia en instrumenten voor immunologie, celbiologie en moleculaire biologie.

Colcemid [477-30-5]

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Referentie NB-64-09556-25mg

Formaat : 25mg

Merk : Neo Biotech

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Colcemid

(Synonyms: NSC-3096, Kolchicin, Demecolcine, C-12669, C12669, C 12669) Copy Product Info

Synonyms: NSC-3096, Kolchicin, Demecolcine, C-12669, C12669, C 12669

Catalog No. T19720 Copy Product Info

Purity: 99.83%

Colcemid (NSC-3096) is a microtubule polymerization inhibitor that primarily targets tubulin, with an IC₅₀ of 2.4 μM. Demecolcine interacts with tubulin dimers, induces antimitotic effects, and inhibits microtubule growth. Colcemid is a classic reagent used in karyotyping, banding techniques, and cell cycle synchronization. Colcemid can induce apoptosis and is used in studies of tumor and embryonic cloning.

Cas No. 477-30-5

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.83%

Appearance:Solid

Color:White to Yellow

Product Information

Colcemid AI Summary

Colcemid demonstrates significant bioactivity and pharmacological properties across various assays. It inhibits the polymerization of purified bovine brain tubulin with an IC50 of 2400.0 nM and displaces [3H]colchicine from tubulin with an IC50 of 3580.0 nM. The compound shows potent cytotoxicity against multiple human cancer cell lines, including MCF7, H460, and SF-268, with EC50 values of 600.0 nM, 690.0 nM, and 6200.0 nM, respectively. Additionally, it exhibits cytotoxicity against KB cells with an ED50 of less than 0.25 µg/ml. Colcemid also causes cell cycle arrest at the G2/M phase in human MCF7 and HeLa cells, indicative of its potential as an antimitotic agent. In vivo, Colcemid shows effectiveness against lymphocytic leukemia P388 in mice, with a potency of 10.8 µM/kg and toxicity values leading to an LD50 of 236.7 µM/kg after a single intramuscular injection. It also exhibits acute toxicity in mice with an LD50 of 237.0 mg/kg. In terms of lipophilicity, it has a partition coefficient (logP) value of 1.53 and logD value of 1.37 at pH 7.4. Moreover, the compound has no significant antimicrobial activity against Micrococcus luteus, Mycobacterium smegmatis, Saccharomyces cerevisiae, and Aspergillus niger, with MIC values greater than 500 µg/ml. Finally, Colcemid shows activation of human and rat pregnane X receptor (PXR), with competitive binding affinity to the human PXR ligand-binding domain (LBD), suggesting moderate potency in these assays. It also affects mRNA translation, nuclear factor kappa B (NF-kB) activation, and induces an irreversible mitotic block in human A2780S cells..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Colcemid (NSC-3096) is a microtubule polymerization inhibitor that primarily targets tubulin, with an IC₅₀ of 2.4 μM. Demecolcine interacts with tubulin dimers, induces antimitotic effects, and inhibits microtubule growth. Colcemid is a classic reagent used in karyotyping, banding techniques, and cell cycle synchronization. Colcemid can induce apoptosis and is used in studies of tumor and embryonic cloning.
Targets & IC50	tubulin:2.4 μM (EC50), HepG2 cells:0.09 μM (EC50), MCF-7 cells:0.6 μM (EC50), SF-268 cells:6.2 μM (EC50), NCI-H460 cells:0.69 μM (EC50)
In vitro	Methods: In a co-culture system of neonatal rat cardiomyocytes (CM) and myofibroblasts (MF), mitochondria were labeled with Ad-Mito-EGFP. After 4-hour treatment with Colcemid (4 μM), live cells were tracked in real-time using confocal microscopy to monitor mitochondrial movement within MNTs, and migration velocity was calculated. Results: Colcemid treatment significantly reduced mitochondrial migration speed within MNTs but did not affect MNT formation. [1]
Synonyms	NSC-3096, Kolchicin, Demecolcine, C-12669, C12669, C 12669

Chemical Properties

Molecular Weight	371.43
Formula	C₂₁H₂₅NO₅
Cas No.	477-30-5
Smiles	COC1=C2C(C([C@H](CCC2=CC(OC)=C1OC)NC)=C3)=CC=C(OC)C3=O
Relative Density.	1.21g/cm3

Storage & Solubility Information

Storage

Keep away from moisture,Store at low temperature Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 55 mg/mL (148.08 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (5.38 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.6923 mL	13.4615 mL	26.9230 mL	134.6149 mL
5 mM	0.5385 mL	2.6923 mL	5.3846 mL	26.9230 mL
10 mM	0.2692 mL	1.3461 mL	2.6923 mL	13.4615 mL
20 mM	0.1346 mL	0.6731 mL	1.3461 mL	6.7307 mL
50 mM	0.0538 mL	0.2692 mL	0.5385 mL	2.6923 mL
100 mM	0.0269 mL	0.1346 mL	0.2692 mL	1.3461 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

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