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> Collagen Type I

Collagen Type I

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Referentie C7510-17K-200ug

Formaat : 200ug

Merk : US Biological


C7510-17K Collagen Type I

Clone Type
Polyclonal
Host
goat
Source
human
Swiss Prot
P02452
Isotype
IgG
Grade
Affinity Purified
Applications
DB E IC IHC
Crossreactivity
Hu
Accession #
NP_000079
Shipping Temp
Blue Ice
Storage Temp
-20°C

Type I collagen is the most common of the collagens in vertebrates. It comprises up to 90% of the skeletons of the mammals and is also widespread all over the body. Such as: skin, tendons, ligaments, cornea, intervertebral disks, dentine, arteries and granulation tissues as the main locations. The importance of type I collagen for medical research is that it is involved in many human and animal diseases, including fibrosis, osteoporosis, cancer, atherosclerosis etc. Type I
collagen is frequently utilized to monitor physiological changes in tissues as well as being used as diagnostic tools in various pathological conditions.

Applications:
Suitable for use in Dot and Slot Blot, ELISA, Indirect Immunohistochemistry and Indirect Immunocytochemistry. Not recommended for paraffin tissue sections. Other applications not tested.

Recommended Dilutions:
Dot and Slot Blot: 1:100-1:500
ELISA: 1:50-1:100
Immunohistochemistry (Indirect, frozen sections only): 1:10-1:20
Immunocytochemistry (Indirect): 1:10-1:20
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Pab|Isotype: IgG|Host: goat|Source: human|Concentration: ~0.5mg/ml|Form: Supplied as a liquid in 100mM borate buffered saline, pH 8.2. No preservatives added.|Purity: Purified by affinity chromatography. |Immunogen: Human placental collagen type I isolated by limited pepsin digestion and selective salt precipitation.|Specificity: Recognizes human type I collagen. Crossreactivity (ELISA): <10% collagen types II, III, IV, V and VI. May show reactivity to type I collagen from other species. Has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Human placental collagen type I isolated by limited pepsin digestion and selective salt precipitation.
Form
Supplied as a liquid in 100mM borate buffered saline, pH 8.2. No preservatives added.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human type I collagen. Crossreactivity (ELISA): <10% collagen types II, III, IV, V and VI. May show reactivity to type I collagen from other species. Has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).