Cortisol 3-CMO (BSA)

Synonyms:
Hydrocortisone-3-CMO-BSA; 4-Pregnen-11b,17,21-triol-3,20-dione 3-Carboxymethyloxime-BSA
11b, 17a, 21-Trihydroy-4-Pregnene-3, 20-dione 3-(0-carboxymethyl)oxime

Molecular Weight:
65,000 + (xS)

Purity:
One spot (homogenous) when tested by TLC.

Appearance:
Supplied as a lyophilized powder from ddH2O.

Molar Ratio (Steroid : BSA)
As Reported

Solubility of BSA Conjugates:
Haptens with ~30 steroids per molecule are designed for raising antibodies. They are virtually insoluble in water and buffer without detergents. The lack of solubility in water arises from both the steroid content and crosslinking. We suggest solubilization with a detergent with vigorous stirring, for example Freund's adjuvant (which contains a detergent) works well. Place the steroid onto the solution, not the other way round. Dissolving BSA conjugates in water for cell culture experiments with molar ratios of greater than 10 moles of steroid per mole of BSA is almost impossible.

Storage and Stabilty:
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute and aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.